Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sorcin (SRI) Standard Curve.)
Mouse Sorcin (SRI) ELISA Kit | SRI elisa kit
Mouse Sorcin (SRI) ELISA Kit
Gene Names
Sri; Sor; 2210417O06Rik; 2900070H08Rik
Reactivity
Mouse
Synonyms
Sorcin (SRI); Mouse Sorcin (SRI) ELISA Kit; SRI; FLJ26259; SCN; H_RG167B05.1; calcium binding protein amplified in mutlidrug-resistant cells; SRI elisa kit
Reactivity
Mouse
Specificity
Mouse Sorcin (SRI) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SRI. No significant cross-reactivity or interference between Mouse SRI and analogues was observed.
Sequence Length
198
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sorcin (SRI) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Sorcin (SRI) Standard Curve.)
Related Product Information for SRI elisa kit
Description: This Mouse Sorcin (SRI) ELISA Kit employs a two-site sandwich ELISA to quantitate SRI in samples. An antibody specific for SRI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SRI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sarcalumenin is a gene which encodes a 160-kD glycoprotein protein involved in calcium signaling.Muscle contraction is triggered by the release of calcium from the sarcoplasmic reticulum, whereas muscle relaxation is achieved by rapid reuptake of calcium from the cytosol into the lumen of the sarcoplasmic reticulum. Sequestration of calcium in the lumen of the sarcoplasmic reticulum is also an essential step in the overall contraction-relaxation cycle of muscle cells. The rabbit sarcoplasmic reticulum contains 2 immunochemically related glycoproteins with apparent molecular masses of 53 and 160 kD.The deduced 160-kD glycoprotein contains an N-terminal signal sequence and a 453-amino acid C-terminal sequence that together form a protein that is identical to the 53-kD glycoprotein.
Background: Sarcalumenin is a gene which encodes a 160-kD glycoprotein protein involved in calcium signaling.Muscle contraction is triggered by the release of calcium from the sarcoplasmic reticulum, whereas muscle relaxation is achieved by rapid reuptake of calcium from the cytosol into the lumen of the sarcoplasmic reticulum. Sequestration of calcium in the lumen of the sarcoplasmic reticulum is also an essential step in the overall contraction-relaxation cycle of muscle cells. The rabbit sarcoplasmic reticulum contains 2 immunochemically related glycoproteins with apparent molecular masses of 53 and 160 kD.The deduced 160-kD glycoprotein contains an N-terminal signal sequence and a 453-amino acid C-terminal sequence that together form a protein that is identical to the 53-kD glycoprotein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sorcin isoform 1
NCBI Official Synonym Full Names
sorcin
NCBI Official Symbol
Sri
NCBI Official Synonym Symbols
Sor; 2210417O06Rik; 2900070H08Rik
NCBI Protein Information
sorcin
UniProt Protein Name
Sorcin
UniProt Gene Name
Sri
Uniprot Description
SRI: Calcium-binding protein that modulates excitation- contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 5 A1|5 3.38 cM
Cellular Component: axon; axon terminus; cytoplasm; cytosol; endoplasmic reticulum membrane; intracellular; membrane; mitochondrion; nucleoplasm; plasma membrane; sarcoplasmic reticulum; smooth endoplasmic reticulum; T-tubule; vesicle; Z disc
Molecular Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; identical protein binding; metal ion binding; protease binding; protein binding; protein heterodimerization activity
Biological Process: calcium ion transport; cytoplasmic sequestering of transcription factor; negative regulation of heart rate; positive regulation of release of sequestered calcium ion into cytosol; proteolysis; regulation of calcium ion transport
Research Articles on SRI
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Product Notes
The Mouse SRI sri (Catalog #AAA9716864) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716864 ELISA Kit recognizes Mouse SRI. It is sometimes possible for the material contained within the vial of "Sorcin (SRI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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