Typical Testing Data/Standard Curve (for reference only)
Mouse Slc18a2 ELISA Kit | Slc18a2 elisa kit
Mouse Slc18a2(Solute Carrier Family 18,Member 2)ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Slc18a2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Slc18a2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Slc18a2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Slc18a2. You can calculate the concentration of Mouse Slc18a2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The vesicular monoamine transporter acts to accumulate cytosolic monoamines into synaptic vesicles, using the proton gradient maintained across the synaptic vesicular membrane. Its proper function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders. The transporter is a site of action of important drugs, including reserpine and tetrabenazine (summary by Peter et al., 1993 [PubMed 7905859]). See also SLC18A1 (MIM 193002).[supplied by OMIM, Jan 2011]
Uniprot Description
SLC18A2: Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis. Belongs to the major facilitator superfamily. Vesicular transporter family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter
Chromosomal Location of Human Ortholog: 10q25
Cellular Component: synaptic vesicle; membrane; cell soma; synaptic vesicle membrane; integral to plasma membrane; plasma membrane; terminal button
Molecular Function: serotonin transmembrane transporter activity; enzyme binding; amine transmembrane transporter activity; heat shock protein binding; monoamine transmembrane transporter activity; drug binding
Biological Process: dopamine transport; amine transport; neurotransmitter secretion; death; monoamine transport; response to amphetamine; locomotory behavior; response to herbicide; response to cocaine; glucose homeostasis; sequestering of neurotransmitter; endocytic recycling; post-embryonic development; response to starvation; synaptic transmission; response to zinc ion; insulin secretion; serotonin transport; transmembrane transport; negative regulation of neurotransmitter transport; response to corticosterone stimulus; aging
Research Articles on Slc18a2
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Product Notes
The Mouse Slc18a2 slc18a2 (Catalog #AAA2515991) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515991 ELISA Kit recognizes Mouse Slc18a2. It is sometimes possible for the material contained within the vial of "Slc18a2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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