SDS-PAGE
ACY1 / Aminoacylase-1 Recombinant Protein | ACY1 recombinant protein
Recombinant Mouse ACY1 / Aminoacylase-1 Protein (His tag)
Gene Names
Acy1; Acy-1; 1110014J22Rik
Purity
> 95 % as determined by SDS-PAGE
Synonyms
ACY1 / Aminoacylase-1; Recombinant Mouse ACY1 / Aminoacylase-1 Protein (His tag); 1110014J22Rik; Acy-1; Acy1; ACY1 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
408
Application Notes
The recombinant mouse ACY1 consists of 402 amino acids and has a calculated molecular mass of 45 kDa as estimated by SDS-PAGE under reducing conditions.
Predicted N Terminal
Gln 18
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for ACY1 recombinant protein
Background: Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
Description: A DNA sequence encoding the mouse ACY1 (Q99JW2) (Met 1-Ser 408) was expressed with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the mouse ACY1 (Q99JW2) (Met 1-Ser 408) was expressed with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,781 Da
NCBI Official Full Name
aminoacylase-1
NCBI Official Synonym Full Names
aminoacylase 1
NCBI Official Symbol
Acy1
NCBI Official Synonym Symbols
Acy-1; 1110014J22Rik
NCBI Protein Information
aminoacylase-1
UniProt Protein Name
Aminoacylase-1
Protein Family
UniProt Gene Name
Acy1
UniProt Synonym Gene Names
ACY-1
Uniprot Description
ACY1: Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). Defects in ACY1 are the cause of aminoacylase-1 deficiency (ACY1D). ACY1D results in a metabolic disorder manifesting with encephalopathy, unspecific psychomotor delay, psychomotor delay with atrophy of the vermis and syringomyelia, marked muscular hypotonia or normal clinical features. Epileptic seizures are a frequent feature. All affected individuals exhibit markedly increased urinary excretion of several N-acetylated amino acids. Belongs to the peptidase M20A family.
Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.1.14; Hydrolase
Chromosomal Location of Human Ortholog: 9 F1|9 57.49 cM
Molecular Function: aminoacylase activity; zinc ion binding
Biological Process: peptide catabolic process; proteolysis
Similar Products
Product Notes
The ACY1 acy1 (Catalog #AAA2547218) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant mouse ACY1 consists of 402 amino acids and has a calculated molecular mass of 45 kDa as estimated by SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the ACY1 acy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACY1 / Aminoacylase-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
