Mouse Sex Hormone Binding Globulin (SHBG) ELISA Kit | SHBG elisa kit

Mouse Sex Hormone Binding Globulin (SHBG) Mini Samples ELISA Kit

Cat. Num. AAA2708294

• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Sex Hormone Binding Globulin (SHBG); Mouse Sex Hormone Binding Globulin (SHBG) Mini Samples ELISA Kit; Sex Hormone Binding Globulin; ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin; SHBG elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Sex Hormone Binding Globulin (SHBG).; No significant cross-reactivity or interference between Mini Samples Sex Hormone Binding Globulin (SHBG) and analogues was observed.
• Sequence Length: 403

• Applicable Applications for SHBG elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.121ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Sex Hormone Binding Globulin (SHBG) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Sex Hormone Binding Globulin (SHBG) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Sex Hormone Binding Globulin (SHBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Sex Hormone Binding Globulin (SHBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Sex Hormone Binding Globulin (SHBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Sex Hormone Binding Globulin (SHBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 6755506
• NCBI Accession #: NP_035497.1
• NCBI GenBank Nucleotide #: NP_035497.1
• UniProt Accession #: P97497
• NCBI Official Full Name: sex hormone-binding globulin
• UniProt Protein Name: Sex hormone-binding globulin
• Protein Family: Sex hormone-binding globulin
• UniProt Gene Name: Shbg
• UniProt Synonym Gene Names: SHBG; SBP; ABP

Uniprot Description

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration ().