Flow Cytometry (FC/FACS)
(Staining of ConA stimulated splenocytes with 0.25 ug of CD3 antibody (PE) and 0.5 ug of Rat IgG2a kappa Isotype Control (FITC) (left) or 0.25 ug of Ly-6A/E antibody (FITC) (right). Total viable cells were used for analysis.)
Rat Ly6A/E Monoclonal Antibody | anti-Ly6A/E antibody
Ly6A/E antibody (FITC)
Applications
Flow Cytometry, Functional Assay
Synonyms
Ly6A/E; Monoclonal Antibody; Ly6A/E antibody (FITC); Monoclonal Ly6A/E; Anti-Ly6A/E; Sca1; LyA/E-6; Ly6E; Ly6AE; LyA/E 6; Ly6A; anti-Ly6A/E antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
D7
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-Ly6A/E antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.125 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Biological Significance
The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.
Tag/Conjugate
FITC
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of ConA stimulated splenocytes with 0.25 ug of CD3 antibody (PE) and 0.5 ug of Rat IgG2a kappa Isotype Control (FITC) (left) or 0.25 ug of Ly-6A/E antibody (FITC) (right). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of ConA stimulated splenocytes with 0.25 ug of CD3 antibody (PE) and 0.5 ug of Rat IgG2a kappa Isotype Control (FITC) (left) or 0.25 ug of Ly-6A/E antibody (FITC) (right). Total viable cells were used for analysis.)
Related Product Information for anti-Ly6A/E antibody
Rat monoclonal Ly6A/E antibody (FITC)
Product Categories/Family for anti-Ly6A/E antibody
Similar Products
Product Notes
The Ly6A/E (Catalog #AAA833394) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ly6A/E can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.125 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the Ly6A/E for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly6A/E, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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