Rabbit anti-Human Cyclin C Polyclonal Antibody | anti-CCNC antibody

Cyclin C, CT (Cyclin-C, hSRB11, CCNC, SRB11 Homolog) (AP)

Cat. Num. AAA6471185

• Gene Names: CCNC; CycC; SRB11; hSRB11
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Cyclin C; Polyclonal Antibody; CT (Cyclin-C; hSRB11; CCNC; SRB11 Homolog) (AP); anti-CCNC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCNC.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
• Sequence Length: 283

• Applicable Applications for anti-CCNC antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CCNC (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CCNC Antibody (C-term) in HL60 cell line lysates (35ug/lane). CCNC(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CCNC antibody

Antibodies; Cyclin

References

1. Katona, R.L., Acta. Biol. Hung. 58(1), 133-137 (2007). 2. Ohata, N., Int. J. Mol. Med. 18(6), 1153-1158 (2006). 3. Sinkkonen, L., Nucleic Acids Res. 33(8), 2440-2451 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 61676091
• NCBI GeneID: 892
• NCBI Accession #: NP_005181
• NCBI GenBank Nucleotide #: NP_005181.2
• UniProt Accession #: P24863
• Molecular Weight: 33.243
• NCBI Official Full Name: cyclin-C isoform a
• NCBI Official Synonym Full Names: cyclin C
• NCBI Official Symbol: CCNC
• NCBI Official Synonym Symbols: CycC; SRB11; hSRB11
• NCBI Protein Information: cyclin-C
• UniProt Protein Name: Cyclin-C
• Protein Family: Cyclin
• UniProt Gene Name: CCNC
• UniProt Synonym Gene Names: hSRB11
• UniProt Entry Name: CCNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNC: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Belongs to the cyclin family. Cyclin C subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; Srb-mediator complex; DNA-directed RNA polymerase II, holoenzyme

Molecular Function: protein binding; protein kinase binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity