Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using NMUR1 Antibody.)
Rabbit NMUR1 Polyclonal Antibody | anti-NMUR1 antibody
NMUR1 Antibody
Gene Names
NMUR1; FM3; FM-3; GPC-R; GPR66; NMU1R; (FM-3)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NMUR1; Polyclonal Antibody; NMUR1 Antibody; FM-3; G protein-coupled receptor 66; G-protein coupled receptor 66; G-protein coupled receptor FM-3; GPR66; Growth hormone secretagogue receptor family Member 3; Neuromedin U Receptor 1; Neuromedin-U receptor 1; NMU-R1; NMUR1_HUMAN; anti-NMUR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NMUR1 antibody detects endogenous levels of total NMUR1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NMUR1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:200
IF/ICC: 1:100-1:500
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human NMUR1, corresponding to a region within N-terminal amino acids.
Function
Receptor for the neuromedin-U and neuromedin-S neuropeptides.
Similarity
Belongs to the G-protein coupled receptor 1 family.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using NMUR1 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using NMUR1 Antibody.)
Immunohistochemistry (IHC)
(MBS9602176 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602176 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence/Immunocytochemistry (IF/ICC)
(MBS9602176 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF9133 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
Immunofluorescence/Immunocytochemistry (IF/ICC)
(MBS9602176 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF9133 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48 kDa
NCBI Official Full Name
neuromedin-U receptor 1
NCBI Official Synonym Full Names
neuromedin U receptor 1
NCBI Official Symbol
NMUR1
NCBI Official Synonym Symbols
FM3; FM-3; GPC-R; GPR66; NMU1R; (FM-3)
NCBI Protein Information
neuromedin-U receptor 1
UniProt Protein Name
Neuromedin-U receptor 1
Protein Family
UniProt Gene Name
NMUR1
UniProt Synonym Gene Names
GPR66; NMU-R1
Uniprot Description
Receptor for the neuromedin-U and neuromedin-S neuropeptides.
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Product Notes
The NMUR1 nmur1 (Catalog #AAA9602176) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NMUR1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NMUR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:1000 IHC: 1:200 IF/ICC: 1:100-1:500 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the NMUR1 nmur1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMUR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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