Mouse Secretogranin II (SCG2) ELISA Kit | SCG2 elisa kit

Mouse Secretogranin II (SCG2) ELISA Kit

Cat. Num. AAA2021597

• Reactivity: Mouse
• Synonyms: Secretogranin II (SCG2); Mouse Secretogranin II (SCG2) ELISA Kit; SN; SgII; CHGC; Chromogranin C; Secretoneurin; SCG2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secretogranin II (SCG2).; No significant cross-reactivity or interference between Secretogranin II (SCG2) and analogues was observed.
• Sequence Length: 617

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretogranin II (SCG2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretogranin II (SCG2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCG2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretogranin II (SCG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretogranin II (SCG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretogranin II (SCG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretogranin II (SCG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SCG2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 338051
• Molecular Weight: 70,941 Da
• NCBI Official Full Name: secretogranin II
• UniProt Protein Name: Secretogranin-2
• Protein Family: Secretogranin
• UniProt Gene Name: SCG2
• UniProt Synonym Gene Names: CHGC; SgII; SN
• UniProt Entry Name: SCG2_HUMAN

Uniprot Description

SCG2: Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. Belongs to the chromogranin/secretogranin protein family.

Protein type: Cytokine; Secreted; Apoptosis; Motility/polarity/chemotaxis; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2q35-q36

Cellular Component: extracellular space; secretory granule

Molecular Function: cytokine activity; chemoattractant activity

Biological Process: positive chemotaxis; negative regulation of endothelial cell proliferation; eosinophil chemotaxis; protein secretion; MAPKKK cascade; endothelial cell migration; positive regulation of endothelial cell proliferation; angiogenesis; inflammatory response; induction of positive chemotaxis