Bovine Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit | HSP90b1 elisa kit

Cattle Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit

Cat. Num. AAA2702544

• Gene Names: HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• Reactivity: Bovine
• Synonyms: Heat Shock Protein 90kDa Beta 1 (HSP90b1); Cattle Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit; HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen(gp96)1;94 kDa glucose-regulated protein; Endoplasmin; HSP90b1 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90kDa Beta 1 (HSP90b1).; No significant cross-reactivity or interference between Heat Shock Protein 90kDa Beta 1 (HSP90b1) and analogues was observed.
• Sequence Length: 803

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP90b1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Beta 1 (HSP90b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Beta 1 (HSP90b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119618130
• NCBI GeneID: 7184
• UniProt Accession #: P14625; Q96A97
• Molecular Weight: 92,469 Da
• NCBI Official Full Name: heat shock protein 90kDa beta (Grp94), member 1, isoform CRA_b
• NCBI Official Synonym Full Names: heat shock protein 90 beta family member 1
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
• NCBI Protein Information: endoplasmin
• UniProt Protein Name: Endoplasmin
• Protein Family: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94

NCBI Description

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Uniprot Description

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.