Mouse Secretagogin (SCGN) ELISA Kit | SCGN elisa kit

Mouse Secretagogin (SCGN) ELISA Kit

Cat. Num. AAA2022064

• Gene Names: SCGN; SEGN; CALBL; SECRET; setagin; DJ501N12.8
• Reactivity: Mouse
• Synonyms: Secretagogin (SCGN); Mouse Secretagogin (SCGN) ELISA Kit; SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin; EF-Hand Calcium Binding Protein; SCGN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secretagogin (SCGN).; No significant cross-reactivity or interference between Secretagogin (SCGN) and analogues was observed.
• Sequence Length: 276

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.4pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretagogin (SCGN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretagogin (SCGN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCGN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretagogin (SCGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretagogin (SCGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretagogin (SCGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretagogin (SCGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SCGN elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 15055537
• NCBI GeneID: 10590
• NCBI Accession #: NP_008929.2
• NCBI GenBank Nucleotide #: NM_006998.3
• UniProt Accession #: O76038; Q5VV44; Q96QV7; Q9UJF6; A8K0B2
• Molecular Weight: 32,040 Da
• NCBI Official Full Name: secretagogin
• NCBI Official Synonym Full Names: secretagogin, EF-hand calcium binding protein
• NCBI Official Symbol: SCGN
• NCBI Official Synonym Symbols: SEGN; CALBL; SECRET; setagin; DJ501N12.8
• NCBI Protein Information: secretagogin
• UniProt Protein Name: Secretagogin
• Protein Family: Secretagogin
• UniProt Gene Name: SCGN
• UniProt Synonym Gene Names: SECRET
• UniProt Entry Name: SEGN_HUMAN

NCBI Description

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

SCGN:

Chromosomal Location of Human Ortholog: 6p22.3-p22.1

Cellular Component: cytoplasm; extracellular region

Molecular Function: calcium ion binding