Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Rabbit DIAPH1 Polyclonal Antibody | anti-DIAPH1 antibody
DIAPH1 Antibody
Gene Names
DIAPH1; DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
DIAPH1; Polyclonal Antibody; DIAPH1 Antibody; Affinity Purified Rabbit Polyclonal DIAPH1 Antibody; Polyclonal DIAPH1 antibody; Anti-DIAPH1 antibody; DFNA1 antibody; DIAPH-1; DIAPH 1; DIAPH-1 antibody; diaphanous-related formin-1 antibody; FLJ25265 antibody; DRF1 antibody; hDIA1 antibody; DIAPH1 antibody; DIAPH 1 antibody; protein diaphanous homolog 1 antibody; DIA1 antibody; LFHL1 antibody; diaphanous-related formin 1 antibody; anti-DIAPH1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.69 mg/ml (varies by lot)
Sequence Length
1262
Applicable Applications for anti-DIAPH1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:1000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Immunogen
DIAPH1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 237 of DIAPH1
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DIAPH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded gastric tissue using DIAPH1 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded gastric tissue using DIAPH1 antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: H1299; B: Raji) using a 7.5% SDS PAGE gel and DIAPH1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: H1299; B: Raji) using a 7.5% SDS PAGE gel and DIAPH1 antibody at a dilution of 1:1000)
Related Product Information for anti-DIAPH1 antibody
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear.
Product Categories/Family for anti-DIAPH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
141 kDa
NCBI Official Full Name
DIAPH1 protein
NCBI Official Synonym Full Names
diaphanous related formin 1
NCBI Official Symbol
DIAPH1
NCBI Official Synonym Symbols
DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1
NCBI Protein Information
protein diaphanous homolog 1
Protein Family
NCBI Description
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Articles on DIAPH1
Similar Products
Product Notes
The DIAPH1 (Catalog #AAA5311841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DIAPH1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:1000 ICC: 1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the DIAPH1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIAPH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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