Mouse Rhodopsin (RHO) ELISA Kit | RHO elisa kit

Mouse Rhodopsin (RHO) ELISA Kit

Cat. Num. AAA2704676

• Gene Names: RHO; RP4; OPN2; CSNBAD1
• Reactivity: Mouse
• Synonyms: Rhodopsin (RHO); Mouse Rhodopsin (RHO) ELISA Kit; OPN2; RP4; Opsin 2; Rod Pigment; Retinitis Pigmentosa 4; Visual Purple; RHO elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rhodopsin (RHO).; No significant cross-reactivity or interference between Rhodopsin (RHO) and analogues was observed.
• Sequence Length: 348

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rhodopsin (RHO) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rhodopsin (RHO) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RHO elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rhodopsin (RHO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rhodopsin (RHO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rhodopsin (RHO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rhodopsin (RHO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506527
• NCBI GeneID: 6010
• NCBI Accession #: NP_000530.1
• NCBI GenBank Nucleotide #: NM_000539.3
• UniProt Accession #: P08100; Q16414; Q2M249
• Molecular Weight: 38,893 Da
• NCBI Official Full Name: rhodopsin
• NCBI Official Synonym Full Names: rhodopsin
• NCBI Official Symbol: RHO
• NCBI Official Synonym Symbols: RP4; OPN2; CSNBAD1
• NCBI Protein Information: rhodopsin
• UniProt Protein Name: Rhodopsin
• Protein Family: Rhodopsin
• UniProt Gene Name: RHO
• UniProt Synonym Gene Names: OPN2

NCBI Description

The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017]

Uniprot Description

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.