Typical Testing Data/Standard Curve (for reference only)
Rabbit Rhodopsin (RHO) ELISA Kit | RHO elisa kit
Rabbit Rhodopsin (RHO) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: RGS9BP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-RGS9BP antibody and an RGS9BP-HRP conjugate. The assay sample and buffer are incubated together with RGS9BP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RGS9BP concentration since RGS9BP from samples and RGS9BP-HRP conjugate compete for the anti-RGS9BP antibody binding site. Since the number of sites is limited, as more sites are occupied by RGS9BP from the sample, fewer sites are left to bind RGS9BP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RGS9BP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
Uniprot Description
Rhodopsin: a G-protein coupled receptor. The light-absorbing visual pigment in Rod photoreceptor cells. Mediates vision in dim light. Consists of the apoprotein, opsin, covalently linked to cis-retinal. Defects in RHO are a cause of autosomal retinitis pigmentosa and congenital stationary night blindness.
Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q21-q24
Cellular Component: Golgi membrane; Golgi apparatus; photoreceptor inner segment; photoreceptor outer segment; integral to plasma membrane; plasma membrane; intercellular junction; rough endoplasmic reticulum membrane
Molecular Function: G-protein coupled receptor activity; protein binding; spectrin binding; metal ion binding; photoreceptor activity; retinal binding
Biological Process: rhodopsin mediated signaling; phototransduction, visible light; G-protein coupled receptor protein signaling pathway; red, far-red light phototransduction; regulation of rhodopsin mediated signaling; visual perception; retina development in camera-type eye; organelle organization and biogenesis; retinoid metabolic process; protein-chromophore linkage; protein amino acid phosphorylation
Disease: Fundus Albipunctatus; Night Blindness, Congenital Stationary, Autosomal Dominant 1; Retinitis Pigmentosa 4
Research Articles on RHO
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Product Notes
The Rabbit RHO rho (Catalog #AAA7224649) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7224649 ELISA Kit recognizes Rabbit RHO. It is sometimes possible for the material contained within the vial of "Rhodopsin (RHO), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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