Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse RAD51-associated protein 1 (RAD51AP1) Standard Curve.)
Mouse RAD51-Associated Protein 1 (RAD51AP1) ELISA Kit | RAD51AP1 elisa kit
Mouse RAD51-Associated Protein 1 (RAD51AP1) ELISA Kit
Gene Names
Rad51ap1; RAB22; 2510006L10Rik
Reactivity
Mouse
Synonyms
RAD51-Associated Protein 1 (RAD51AP1); Mouse RAD51-Associated Protein 1 (RAD51AP1) ELISA Kit; RAD51AP1; PIR51; RAD51-interacting protein; RAD51AP1 elisa kit
Reactivity
Mouse
Specificity
Mouse RAD51-associated protein 1 (RAD51AP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RAD51AP1. No significant cross-reactivity or interference between Mouse RAD51AP1 and analogues was observed.
Sequence Length
337
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse RAD51-associated protein 1 (RAD51AP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse RAD51-associated protein 1 (RAD51AP1) Standard Curve.)
Related Product Information for RAD51AP1 elisa kit
Description: This Mouse RAD51-associated protein 1 (RAD51AP1) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51AP1 in samples. An antibody specific for RAD51AP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAD51AP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD51AP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD51AP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RAD51AP1 specifically stimulated joint molecule formation through structure-specific DNA binding and physical contact with RAD51. RAD51AP1 was required to protect HeLa cells from agents that cause DNA double-strand breaks. At the biochemical level, RAD51AP1 had selective affinity for branched DNA structures, and it stimulated D loop formation by RAD51, a pivotal step in recombination. The predicted PIR51 protein has 335 amino acids and a calculated pI of 9.95. The authors suggested that PIR51 is the human homolog of mouse Rab22 since the 2 proteins share 63% sequence identity. Northern blot analysis revealed that PIR51 is expressed as a 2.4-kb mRNA in human testis and thymus, and at lower levels, in colon and small intestine.
Background: RAD51AP1 specifically stimulated joint molecule formation through structure-specific DNA binding and physical contact with RAD51. RAD51AP1 was required to protect HeLa cells from agents that cause DNA double-strand breaks. At the biochemical level, RAD51AP1 had selective affinity for branched DNA structures, and it stimulated D loop formation by RAD51, a pivotal step in recombination. The predicted PIR51 protein has 335 amino acids and a calculated pI of 9.95. The authors suggested that PIR51 is the human homolog of mouse Rab22 since the 2 proteins share 63% sequence identity. Northern blot analysis revealed that PIR51 is expressed as a 2.4-kb mRNA in human testis and thymus, and at lower levels, in colon and small intestine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
RAD51-associated protein 1 isoform 1
NCBI Official Synonym Full Names
RAD51 associated protein 1
NCBI Official Symbol
Rad51ap1
NCBI Official Synonym Symbols
RAB22; 2510006L10Rik
NCBI Protein Information
RAD51-associated protein 1
UniProt Protein Name
RAD51-associated protein 1
Protein Family
UniProt Gene Name
Rad51ap1
UniProt Entry Name
R51A1_MOUSE
Research Articles on RAD51AP1
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Product Notes
The Mouse RAD51AP1 rad51ap1 (Catalog #AAA9718034) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718034 ELISA Kit recognizes Mouse RAD51AP1. It is sometimes possible for the material contained within the vial of "RAD51-Associated Protein 1 (RAD51AP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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