Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) Standard Curve.)
Mouse Pyruvate Dehydrogenase Protein X Component, Mitochondrial (PDHX) ELISA Kit | PDHX elisa kit
Mouse Pyruvate Dehydrogenase Protein X Component, Mitochondrial (PDHX) ELISA Kit
Gene Names
Pdhx; E3bp; Pdx1; AI481367
Reactivity
Mouse
Synonyms
Pyruvate Dehydrogenase Protein X Component; Mitochondrial (PDHX); Mouse Pyruvate Dehydrogenase Protein X Component; Mitochondrial (PDHX) ELISA Kit; PDHX; DLDBP; E3BP; OPDX; PDX1; proX; dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; pyruvate dehydrogenase complex; E3-binding protein subunit; pyruvate dehydrogenase comp; PDHX elisa kit
Reactivity
Mouse
Specificity
Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PDHX. No significant cross-reactivity or interference between Mouse PDHX and analogues was observed.
Sequence Length
501
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) Standard Curve.)
Related Product Information for PDHX elisa kit
Description: This Mouse Pyruvate dehydrogenase protein X component, mitochondrial (PDHX) ELISA Kit employs a two-site sandwich ELISA to quantitate PDHX in samples. An antibody specific for PDHX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDHX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDHX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDHX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 component of the PDH complex. The open reading frame encodes an 501-amino acid protein sequence that is 20% identical with that encoded by the yeast PDX1 gene and 40% identical with that encoded by the lipoacyltransferase component E2 of the PDH (E2), and includes a lipoyl-bearing domain. Sequence comparisons showed that the PDHX protein contains an E3-binding domain.
Background: The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53-kD protein called protein X. Component X binds to the E3 component of the PDH complex. The open reading frame encodes an 501-amino acid protein sequence that is 20% identical with that encoded by the yeast PDX1 gene and 40% identical with that encoded by the lipoacyltransferase component E2 of the PDH (E2), and includes a lipoyl-bearing domain. Sequence comparisons showed that the PDHX protein contains an E3-binding domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyruvate dehydrogenase protein X component, mitochondrial
NCBI Official Synonym Full Names
pyruvate dehydrogenase complex, component X
NCBI Official Symbol
Pdhx
NCBI Official Synonym Symbols
E3bp; Pdx1; AI481367
NCBI Protein Information
pyruvate dehydrogenase protein X component, mitochondrial
UniProt Protein Name
Pyruvate dehydrogenase protein X component, mitochondrial
Protein Family
UniProt Gene Name
Pdhx
UniProt Entry Name
ODPX_MOUSE
Uniprot Description
PDHX: a mitochondrial protein required for anchoring dihydrolipoamide dehydrogenase (E3) to the pyruvate dehydrogenase complexes (PDHC) of eukaryotes. This specific binding is essential for a PDHC function. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and protein X bound by non-covalent bonds. Belongs to the 2-oxoacid dehydrogenase family. Contains 1 lipoyl-binding domain.
Protein type: Mitochondrial
Cellular Component: mitochondrion
Molecular Function: transferase activity, transferring acyl groups
Biological Process: metabolic process
Research Articles on PDHX
Similar Products
Product Notes
The Mouse PDHX pdhx (Catalog #AAA9718061) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718061 ELISA Kit recognizes Mouse PDHX. It is sometimes possible for the material contained within the vial of "Pyruvate Dehydrogenase Protein X Component, Mitochondrial (PDHX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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