* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. It encodes a protein which shows 81% amino acid identity to the mouse Wnt8A protein.WNT8A gene encode a 351-amino acid protein with an N-terminal signal peptide, 3 N-linked glycosylation sites, and residues conserved among members of the WNT family. The WNT8A protein shares 63.2% sequence identity with human WNT8B and homology with the mouse Wnt8d protein.
NCBI and Uniprot Product Information
Uniprot Description
WNT7B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; plasma membrane; extracellular region
Molecular Function: protein binding; frizzled binding; receptor agonist activity; receptor binding
Biological Process: oxygen homeostasis; cell proliferation in forebrain; multicellular organismal development; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; signal transduction; anatomical structure regression; activation of JNK activity; negative regulation of neurogenesis; neuron differentiation; cellular metabolic process; central nervous system vasculogenesis; vasculature development; homeostatic process; positive regulation of cell proliferation; synapse organization and biogenesis; regulation of cell projection size; establishment and/or maintenance of polarity of embryonic epithelium; angiogenesis; neurite development; Wnt receptor signaling pathway; cell fate commitment; in utero embryonic development; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; stem cell development; negative regulation of smoothened signaling pathway; embryonic organ development; smooth muscle cell differentiation; neurite morphogenesis; lung development
Research Articles on WNT7B
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Product Notes
The Mouse WNT7B wnt7b (Catalog #AAA9716616) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716616 ELISA Kit recognizes Mouse WNT7B. It is sometimes possible for the material contained within the vial of "Protein Wnt-7b (WNT7B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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