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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Protein Wnt-7b (WNT7B) Standard Curve.)

Mouse Protein Wnt-7b (WNT7B) ELISA Kit | WNT7B elisa kit

Mouse Protein Wnt-7b (WNT7B) ELISA Kit

Gene Names
Wnt7b; Wnt-7b
Reactivity
Mouse
Synonyms
Protein Wnt-7b (WNT7B); Mouse Protein Wnt-7b (WNT7B) ELISA Kit; WNT7B elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Mouse Protein Wnt-7b (WNT7B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse WNT7B. No significant cross-reactivity or interference between Mouse WNT7B and analogues was observed.
Sequence Length
282
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Protein Wnt-7b (WNT7B) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Protein Wnt-7b (WNT7B) Standard Curve.)
Related Product Information for WNT7B elisa kit
Description: This Mouse Protein Wnt-7b (WNT7B) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT7B in samples. An antibody specific for WNT7B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT7B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT7B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT7B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. It encodes a protein which shows 81% amino acid identity to the mouse Wnt8A protein.WNT8A gene encode a 351-amino acid protein with an N-terminal signal peptide, 3 N-linked glycosylation sites, and residues conserved among members of the WNT family. The WNT8A protein shares 63.2% sequence identity with human WNT8B and homology with the mouse Wnt8d protein.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein Wnt-7b isoform 3
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 7B
NCBI Official Symbol
Wnt7b
NCBI Official Synonym Symbols
Wnt-7b
NCBI Protein Information
protein Wnt-7b
UniProt Protein Name
Protein Wnt-7b
Protein Family
UniProt Gene Name
Wnt7b
UniProt Synonym Gene Names
Wnt-7b
UniProt Entry Name
WNT7B_MOUSE

Uniprot Description

WNT7B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; cytoplasm; plasma membrane; extracellular region

Molecular Function: protein binding; frizzled binding; receptor agonist activity; receptor binding

Biological Process: oxygen homeostasis; cell proliferation in forebrain; multicellular organismal development; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; signal transduction; anatomical structure regression; activation of JNK activity; negative regulation of neurogenesis; neuron differentiation; cellular metabolic process; central nervous system vasculogenesis; vasculature development; homeostatic process; positive regulation of cell proliferation; synapse organization and biogenesis; regulation of cell projection size; establishment and/or maintenance of polarity of embryonic epithelium; angiogenesis; neurite development; Wnt receptor signaling pathway; cell fate commitment; in utero embryonic development; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; stem cell development; negative regulation of smoothened signaling pathway; embryonic organ development; smooth muscle cell differentiation; neurite morphogenesis; lung development

Research Articles on WNT7B

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Product Notes

The Mouse WNT7B wnt7b (Catalog #AAA9716616) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716616 ELISA Kit recognizes Mouse WNT7B. It is sometimes possible for the material contained within the vial of "Protein Wnt-7b (WNT7B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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