Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein S100-A9 (S100A9) Standard Curve.)
Mouse Protein S100-A9 (S100A9) ELISA Kit | S100A9 elisa kit
Mouse Protein S100-A9 (S100A9) ELISA Kit
Gene Names
S100a9; p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
Reactivity
Mouse
Synonyms
Protein S100-A9 (S100A9); Mouse Protein S100-A9 (S100A9) ELISA Kit; S100A9; 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); calgranulin B; S100A9 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein S100-A9 (S100A9) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S100A9. No significant cross-reactivity or interference between Mouse S100A9 and analogues was observed.
Sequence Length
113
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein S100-A9 (S100A9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein S100-A9 (S100A9) Standard Curve.)
Related Product Information for S100A9 elisa kit
Description: This Mouse Protein S100-A9 (S100A9) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A9 in samples. An antibody specific for S100A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: S100 calcium binding protein A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This contributes to creating an overall immunosuppressive microenvironemnt that may contribute to the inability of a protective or therapeutic cellular immune response to be generated by the tumor-bearing host. Outside of malignancy, S100A9 in association with its dimerization partner, S100A8 (MRP8 or calgranulin A) signals for lymphocyte recruitment in sites of inflammation. S100A9/A8 (synonyma: Calgranulin A/B; Calprotectin) are also regarded as marker proteins for a number of inflammatory diseases in humans, especially in rheumatoid arthritis.
Background: S100 calcium binding protein A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This contributes to creating an overall immunosuppressive microenvironemnt that may contribute to the inability of a protective or therapeutic cellular immune response to be generated by the tumor-bearing host. Outside of malignancy, S100A9 in association with its dimerization partner, S100A8 (MRP8 or calgranulin A) signals for lymphocyte recruitment in sites of inflammation. S100A9/A8 (synonyma: Calgranulin A/B; Calprotectin) are also regarded as marker proteins for a number of inflammatory diseases in humans, especially in rheumatoid arthritis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein S100-A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9 (calgranulin B)
NCBI Official Symbol
S100a9
NCBI Official Synonym Symbols
p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
NCBI Protein Information
protein S100-A9
UniProt Protein Name
Protein S100-A9
Protein Family
UniProt Gene Name
S100a9
UniProt Synonym Gene Names
Cagb; Mrp14; MRP-14; p14
UniProt Entry Name
S10A9_MOUSE
Uniprot Description
S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.
Protein type: Calcium-binding
Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; plasma membrane; extracellular region; nucleus
Molecular Function: antioxidant activity; arachidonic acid binding; RAGE receptor binding; zinc ion binding; microtubule binding; metal ion binding; calcium ion binding
Biological Process: caspase activation; neutrophil chemotaxis; immune system process; apoptosis; leukocyte chemotaxis; positive regulation of peptide secretion; chemotaxis; leukocyte migration during inflammatory response; regulation of inflammatory response; actin cytoskeleton reorganization; innate immune response; autophagy; regulation of integrin biosynthetic process; inflammatory response; positive regulation of inflammatory response
Research Articles on S100A9
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Product Notes
The Mouse S100A9 s100a9 (Catalog #AAA9717095) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717095 ELISA Kit recognizes Mouse S100A9. It is sometimes possible for the material contained within the vial of "Protein S100-A9 (S100A9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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