Mouse Prokineticin 2 (PK2) ELISA Kit | PK2 elisa kit

Mouse Prokineticin 2 (PK2) ELISA Kit

Cat. Num. AAA2700028

• Gene Names: PROK2; BV8; HH4; PK2; KAL4; MIT1
• Reactivity: Mouse
• Synonyms: Prokineticin 2 (PK2); Mouse Prokineticin 2 (PK2) ELISA Kit; PK-2; Bv8; MIT1; KAL4; Protein Bv8 Homolog; PK2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prokineticin 2 (PK2).; No significant cross-reactivity or interference between Prokineticin 2 (PK2) and analogues was observed.
• Sequence Length: 129

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 246.91-20000pg/mL
• Sensitivity: < 91.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prokineticin 2 (PK2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prokineticin 2 (PK2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PK2 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Prokineticin 2 (PK2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Prokineticin 2 (PK2) and unlabeled Prokineticin 2 (PK2) (Standards or samples) with the pre-coated antibody specific to Prokineticin 2 (PK2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Prokineticin 2 (PK2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Prokineticin 2 (PK2) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 187167261
• NCBI GeneID: 60675
• NCBI Accession #: NP_001119600.1
• NCBI GenBank Nucleotide #: NM_001126128.1
• UniProt Accession #: Q9HC23; Q53Z79; Q6ISR0
• Molecular Weight: 11,659 Da
• NCBI Official Full Name: prokineticin-2 isoform a
• NCBI Official Synonym Full Names: prokineticin 2
• NCBI Official Symbol: PROK2
• NCBI Official Synonym Symbols: BV8; HH4; PK2; KAL4; MIT1
• NCBI Protein Information: prokineticin-2
• UniProt Protein Name: Prokineticin-2
• Protein Family: Probable serine/threonine-protein kinase
• UniProt Gene Name: PROK2
• UniProt Synonym Gene Names: BV8; PK2

NCBI Description

This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle.