Human Prokineticin 2 (PK2) ELISA Kit | PK2 elisa kit
Human Prokineticin 2 (PK2) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PK2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PK2 and unlabeled PK2 (Standards or samples) with the pre-coated antibody specific to PK2. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PK2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PK2 in the sample.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PROK2: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the AVIT (prokineticin) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p13
Cellular Component: extracellular region
Molecular Function: G-protein-coupled receptor binding
Biological Process: circadian rhythm; activation of MAPK activity; sensory perception of pain; chemotaxis; G-protein coupled receptor protein signaling pathway; cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spermatogenesis; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; negative regulation of apoptosis
Disease: Hypogonadotropic Hypogonadism 4 With Or Without Anosmia
