Typical Testing Data/Standard Curve (for reference only)
Porcine Prokineticin 2 ELISA Kit | PK2 elisa kit
Porcine Prokineticin 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE: This PK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine PK2. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY: PK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PK2 antibody and an PK2-HRP conjugate. The assay sample and buffer are incubated together with PK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PK2 concentration since PK2 from samples and PK2-HRP conjugate compete for the anti-PK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PK2 from the sample, fewer sites are left to bind PK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PK2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions. Mutations in this gene are associated with Kallmann syndrome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PROK2: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the AVIT (prokineticin) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3p13
Cellular Component: extracellular region
Molecular Function: G-protein-coupled receptor binding
Biological Process: circadian rhythm; activation of MAPK activity; chemotaxis; sensory perception of pain; G-protein coupled receptor protein signaling pathway; cell proliferation; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spermatogenesis; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; negative regulation of apoptosis
Disease: Hypogonadotropic Hypogonadism 4 With Or Without Anosmia
Research Articles on PK2
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Product Notes
The Porcine PK2 prok2 (Catalog #AAA740241) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA740241 ELISA Kit recognizes Porcine PK2. It is sometimes possible for the material contained within the vial of "Prokineticin 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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