Mouse Platelet Derived Growth Factor AA (PDGFAA) ELISA Kit | PDGFAA elisa kit

Mouse Platelet Derived Growth Factor AA (PDGFAA) Mini Samples ELISA Kit

Cat. Num. AAA2089520

• Reactivity: Mouse
• Synonyms: Platelet Derived Growth Factor AA (PDGFAA); Mouse Platelet Derived Growth Factor AA (PDGFAA) Mini Samples ELISA Kit; PDGF-AA; PDGFAA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Platelet Derived Growth Factor AA (PDGFAA).; No significant cross-reactivity or interference between Mini Samples Platelet Derived Growth Factor AA (PDGFAA) and analogues was observed.
• Sequence Length: 196

• Assay Type: Double-antibody Sandwich
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Platelet Derived Growth Factor AA (PDGFAA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Platelet Derived Growth Factor AA (PDGFAA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Related Product Information for PDGFAA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Platelet Derived Growth Factor AA (PDGFAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Platelet Derived Growth Factor AA (PDGFAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Platelet Derived Growth Factor AA (PDGFAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Platelet Derived Growth Factor AA (PDGFAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6715566
• NCBI GeneID: 18590
• NCBI Accession #: NP_032834.1
• NCBI GenBank Nucleotide #: NM_008808.3
• UniProt Accession #: P20033
• Molecular Weight: 22,267 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A
• NCBI Official Synonym Full Names: platelet derived growth factor, alpha
• NCBI Official Symbol: Pdgfa
• NCBI Protein Information: platelet-derived growth factor subunit A
• UniProt Protein Name: Platelet-derived growth factor subunit A
• UniProt Gene Name: Pdgfa
• UniProt Synonym Gene Names: PDGF subunit A

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5 G2|5 77.65 cM

Cellular Component: cell surface; extracellular space; microvillus

Molecular Function: collagen binding; growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: actin cytoskeleton organization and biogenesis; alveolus development; angiogenesis; blood coagulation; cell projection biogenesis; gut development; hair follicle development; negative chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; organ morphogenesis; platelet-derived growth factor receptor signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling cascade; regulation of peptidyl-tyrosine phosphorylation; regulation of smooth muscle cell migration; response to wounding; skin development