Mouse PICK1 ELISA Kit | PICK1 elisa kit

Mouse PICK1 (Protein Interacting With Protein Kinase C, Alpha 1) ELISA Kit

Cat. Num. AAA2501754

• Gene Names: PICK1; PICK; PRKCABP
• Reactivity: Mouse
• Synonyms: PICK1; Mouse PICK1 (Protein Interacting With Protein Kinase C; Alpha 1) ELISA Kit; PICK1 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse PICK1. No significant cross-reactivity or interference between Mouse PICK1 and analogues was observed.
• Sequence Length: 415

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PICK1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PICK1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PICK1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PICK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PICK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PICK1. You can calculate the concentration of PICK1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 88758603
• NCBI GeneID: 9463
• NCBI Accession #: NP_001034673.1
• NCBI GenBank Nucleotide #: NM_001039584.1
• UniProt Accession #: Q9NRD5; O95906; B3KS52
• Molecular Weight: 39,808 Da
• NCBI Official Full Name: PRKCA-binding protein
• NCBI Official Synonym Full Names: protein interacting with PRKCA 1
• NCBI Official Symbol: PICK1
• NCBI Official Synonym Symbols: PICK; PRKCABP
• NCBI Protein Information: PRKCA-binding protein; protein interacting with C kinase 1; protein kinase C-alpha-binding protein
• UniProt Protein Name: PRKCA-binding protein
• Protein Family: PRKCA-binding protein
• UniProt Gene Name: PICK1
• UniProt Synonym Gene Names: PRKCABP
• UniProt Entry Name: PICK1_HUMAN

NCBI Description

The protein encoded by this gene contains a PDZ domain, through which it interacts with protein kinase C, alpha (PRKCA). This protein may function as an adaptor that binds to and organizes the subcellular localization of a variety of membrane proteins. It has been shown to interact with multiple glutamate receptor subtypes, monoamine plasma membrane transporters, as well as non-voltage gated sodium channels, and may target PRKCA to these membrane proteins and thus regulate their distribution and function. This protein has also been found to act as an anchoring protein that specifically targets PRKCA to mitochondria in a ligand-specific manner. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PICK1: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ASIC1/ASIC3 channel.

Protein type: Adaptor/scaffold; Ligand, receptor tyrosine kinase; Cell development/differentiation

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: presynaptic membrane; Golgi apparatus; postsynaptic membrane; neuron projection; cytoskeleton; mitochondrion; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: protein C-terminus binding; actin filament binding; protein domain specific binding; identical protein binding; protein binding; enzyme binding; G-protein-coupled receptor binding; protein kinase C binding; metal ion binding; ATPase activity; receptor binding

Biological Process: glial cell development; DNA methylation during gametogenesis; positive regulation of receptor internalization; monoamine transport; receptor clustering; protein amino acid phosphorylation; cellular response to glucose starvation; synaptic transmission; protein kinase C activation; retrograde vesicle-mediated transport, Golgi to ER; DNA methylation during embryonic development; neuronal ion channel clustering; protein targeting