Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxisomal membrane protein 2 (PXMP2) Standard Curve.)
Mouse Peroxisomal Membrane Protein 2 (PXMP2) ELISA Kit | PXMP2 elisa kit
Mouse Peroxisomal Membrane Protein 2 (PXMP2) ELISA Kit
Gene Names
Pxmp2; 22kDa; PMP22
Reactivity
Mouse
Synonyms
Peroxisomal Membrane Protein 2 (PXMP2); Mouse Peroxisomal Membrane Protein 2 (PXMP2) ELISA Kit; PXMP2; FLJ54922; PMP22; PXMP2 elisa kit
Reactivity
Mouse
Specificity
Mouse Peroxisomal membrane protein 2 (PXMP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PXMP2. No significant cross-reactivity or interference between Mouse PXMP2 and analogues was observed.
Sequence Length
193
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxisomal membrane protein 2 (PXMP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peroxisomal membrane protein 2 (PXMP2) Standard Curve.)
Related Product Information for PXMP2 elisa kit
Description: This Mouse Peroxisomal membrane protein 2 (PXMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP2 in samples. An antibody specific for PXMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PXMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PMP22 is a major component of peroxisomal membranes in mammals.It seems to be involved in pore forming activity and may contribute to the unspecific permeability of the organelle membrane. PMP22 is synthesized on free cytosolic ribosomes and then directed to the peroxisome membrane by specific targeting information. Previous studies in rats revealed that PMP22 contains one distinct peroxisomal membrane targeting signal in the amino-terminal cytoplasmic tail. Amino acid sequence alignment of rat and human protein revealed 77% identity including a high conservation of several protein motifs.Human as well as rat PMP22 contains two distinct and nonoverlapping peroxisomal membrane targeting signals, one in the amino-terminal and the other in the carboxyl-terminal protein region.
Background: PMP22 is a major component of peroxisomal membranes in mammals.It seems to be involved in pore forming activity and may contribute to the unspecific permeability of the organelle membrane. PMP22 is synthesized on free cytosolic ribosomes and then directed to the peroxisome membrane by specific targeting information. Previous studies in rats revealed that PMP22 contains one distinct peroxisomal membrane targeting signal in the amino-terminal cytoplasmic tail. Amino acid sequence alignment of rat and human protein revealed 77% identity including a high conservation of several protein motifs.Human as well as rat PMP22 contains two distinct and nonoverlapping peroxisomal membrane targeting signals, one in the amino-terminal and the other in the carboxyl-terminal protein region.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peroxisomal membrane protein 2
NCBI Official Synonym Full Names
peroxisomal membrane protein 2
NCBI Official Symbol
Pxmp2
NCBI Official Synonym Symbols
22kDa; PMP22
NCBI Protein Information
peroxisomal membrane protein 2
UniProt Protein Name
Peroxisomal membrane protein 2
Protein Family
UniProt Gene Name
Pxmp2
UniProt Synonym Gene Names
Pmp22
UniProt Entry Name
PXMP2_MOUSE
Research Articles on PXMP2
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Product Notes
The Mouse PXMP2 pxmp2 (Catalog #AAA9717208) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717208 ELISA Kit recognizes Mouse PXMP2. It is sometimes possible for the material contained within the vial of "Peroxisomal Membrane Protein 2 (PXMP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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