Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear transition protein 2 (TNP2) Standard Curve.)
Mouse Nuclear Transition Protein 2 (TNP2) ELISA Kit | TNP2 elisa kit
Mouse Nuclear Transition Protein 2 (TNP2) ELISA Kit
Gene Names
Tnp2; TP2; Tp-2
Reactivity
Mouse
Synonyms
Nuclear Transition Protein 2 (TNP2); Mouse Nuclear Transition Protein 2 (TNP2) ELISA Kit; TNP2; MGC116783; MGC116785; TP2; TNP2 elisa kit
Reactivity
Mouse
Specificity
Mouse Nuclear transition protein 2 (TNP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNP2. No significant cross-reactivity or interference between Mouse TNP2 and analogues was observed.
Sequence Length
117
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear transition protein 2 (TNP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear transition protein 2 (TNP2) Standard Curve.)
Related Product Information for TNP2 elisa kit
Description: This Mouse Nuclear transition protein 2 (TNP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNP2 in samples. An antibody specific for TNP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TNP2 were not found in human testicular RNA or human testis cDNA libraries. Finding homologous sequences in the human genome was greatly facilitated, however, by the finding that, in the boar and bull genomes, the genes for protamine-1 (PRM1) and Prm2 are clustered with Tnp2. Schluter et al. (1992) tested a genomic human cosmid clone containing the genes for both protamines and found that the TNP2 gene was within a distance of 7.5 kb 3-prime of the PRM2 gene. The gene was transcribed in human testicular RNA only at a level demonstrable by RT-PCR and RNase protection assay. In contrast to the TNP2 genes of mouse, rat, boar, and bull, the human TNP2 gene lacks the nucleotide sequence GCCATCAC in its 3-prime UTR.
Background: TNP2 were not found in human testicular RNA or human testis cDNA libraries. Finding homologous sequences in the human genome was greatly facilitated, however, by the finding that, in the boar and bull genomes, the genes for protamine-1 (PRM1) and Prm2 are clustered with Tnp2. Schluter et al. (1992) tested a genomic human cosmid clone containing the genes for both protamines and found that the TNP2 gene was within a distance of 7.5 kb 3-prime of the PRM2 gene. The gene was transcribed in human testicular RNA only at a level demonstrable by RT-PCR and RNase protection assay. In contrast to the TNP2 genes of mouse, rat, boar, and bull, the human TNP2 gene lacks the nucleotide sequence GCCATCAC in its 3-prime UTR.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nuclear transition protein 2
NCBI Official Synonym Full Names
transition protein 2
NCBI Official Symbol
Tnp2
NCBI Official Synonym Symbols
TP2; Tp-2
NCBI Protein Information
nuclear transition protein 2
UniProt Protein Name
Nuclear transition protein 2
Protein Family
UniProt Gene Name
Tnp2
UniProt Synonym Gene Names
TP-2; TP2
UniProt Entry Name
STP2_MOUSE
Research Articles on TNP2
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Product Notes
The Mouse TNP2 tnp2 (Catalog #AAA9716705) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716705 ELISA Kit recognizes Mouse TNP2. It is sometimes possible for the material contained within the vial of "Nuclear Transition Protein 2 (TNP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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