Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protamine-3 (PRM3) Standard Curve.)
Rat Protamine-3 (PRM3) ELISA Kit | PRM3 elisa kit
Rat Protamine-3 (PRM3) ELISA Kit
Reactivity
Rat
Synonyms
Protamine-3 (PRM3); Rat Protamine-3 (PRM3) ELISA Kit; PRM3; Gene 4; PRM3 elisa kit
Reactivity
Rat
Specificity
Rat Protamine-3 (PRM3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRM3. No significant cross-reactivity or interference between Rat PRM3 and analogues was observed.
Sequence Length
104
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protamine-3 (PRM3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Protamine-3 (PRM3) Standard Curve.)
Related Product Information for PRM3 elisa kit
Description: This Rat Protamine-3 (PRM3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRM3 in samples. An antibody specific for PRM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prm3 encoding a distinctive small acidic protein is present in 13 species from seven orders of mammals. Prm3 gene has not generated retroposons, which supports the contention that genes that are expressed in meiotic and haploid spermatogenic cells do not generate retroposons. The Prm3 mRNA is first detected in early round spermatids, while the PRM3 protein is first detected in late spermatids. Thus, translation of the Prm3 mRNA is developmentally delayed similar to the Prm1, Prm2, and Tnp2 mRNAs. In contrast to PRM1, PRM2, and TNP2, PRM3 is an acidic protein that is localized in the cytoplasm of elongated spermatids and transfected NIH-3T3 cells. To elucidate the function of PRM3, the Prm3 gene was disrupted by homologous recombination.
Background: Prm3 encoding a distinctive small acidic protein is present in 13 species from seven orders of mammals. Prm3 gene has not generated retroposons, which supports the contention that genes that are expressed in meiotic and haploid spermatogenic cells do not generate retroposons. The Prm3 mRNA is first detected in early round spermatids, while the PRM3 protein is first detected in late spermatids. Thus, translation of the Prm3 mRNA is developmentally delayed similar to the Prm1, Prm2, and Tnp2 mRNAs. In contrast to PRM1, PRM2, and TNP2, PRM3 is an acidic protein that is localized in the cytoplasm of elongated spermatids and transfected NIH-3T3 cells. To elucidate the function of PRM3, the Prm3 gene was disrupted by homologous recombination.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protamine-3
NCBI Official Synonym Full Names
protamine 3
NCBI Official Symbol
Prm3
NCBI Protein Information
protamine-3
UniProt Protein Name
Protamine-3
Protein Family
UniProt Gene Name
Prm3
UniProt Entry Name
PRM3_RAT
Similar Products
Product Notes
The Rat PRM3 prm3 (Catalog #AAA9712058) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712058 ELISA Kit recognizes Rat PRM3. It is sometimes possible for the material contained within the vial of "Protamine-3 (PRM3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
