Mouse Neurogenin 3 (NEUROG3) ELISA Kit | NEUROG3 elisa kit

Mouse Neurogenin 3 (NEUROG3) ELISA Kit

Cat. Num. AAA2706419

• Gene Names: NEUROG3; ngn3; Atoh5; NGN-3; Math4B; bHLHa7
• Reactivity: Mouse
• Synonyms: Neurogenin 3 (NEUROG3); Mouse Neurogenin 3 (NEUROG3) ELISA Kit; Math4B; Atoh5; ngn3; bHLHa7; Class A basic helix-loop-helix protein 7; Protein atonal homolog 5; NEUROG3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neurogenin 3 (NEUROG3).; No significant cross-reactivity or interference between Neurogenin 3 (NEUROG3) and analogues was observed.
• Sequence Length: 214

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurogenin 3 (NEUROG3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurogenin 3 (NEUROG3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NEUROG3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurogenin 3 (NEUROG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurogenin 3 (NEUROG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurogenin 3 (NEUROG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurogenin 3 (NEUROG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 306411103
• NCBI GeneID: 50674
• UniProt Accession #: Q9Y4Z2; Q5VVI0; Q6DJX6; Q9BY24
• Molecular Weight: 23,077 Da
• NCBI Official Full Name: neurogenin 3, partial
• NCBI Official Synonym Full Names: neurogenin 3
• NCBI Official Symbol: NEUROG3
• NCBI Official Synonym Symbols: ngn3; Atoh5; NGN-3; Math4B; bHLHa7
• NCBI Protein Information: neurogenin-3
• UniProt Protein Name: Neurogenin-3
• Protein Family: Neurogenin
• UniProt Gene Name: NEUROG3
• UniProt Synonym Gene Names: ATOH5; BHLHA7; NGN3; NGN-3; bHLHa7

NCBI Description

The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).[provided by RefSeq, May 2010]

Uniprot Description

Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types ().