PRMT1 active protein

Human PRMT1 isoform3, 1-353aa, Human, His-MBP tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203118

• Gene Names: PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PRMT1; Human PRMT1 isoform3; 1-353aa; Human; His-MBP tag; E Coli (Bioactivity Validated); HMT1 hnRNP methyltransferase-like 2 isoform 3; ANM1; HCP1; HRMT1L2; IR1B4; protein arginine methyltransferase 1; HMT1 hnRNP methyltransferase-like 2 isoform 3 ANM 1; HCP 1; Heterogeneous nuclear ribonucleoproteins methyltransferase like 2; HMT2; HRMT1 L2; HRMT1L 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like2; HMT 2; HMT1 (hnRNP methyltransferase S; cerevisiae) like 2; HMT1 hnRNP methyltransferase; HMT1 hnRNP methyltransferase like 2; HMT1 hnRNP methyltransferase like 2 (S; cerevisiae); Human mRNA for suppressor for yeast mutant; Human mRNA for suppressor for yeast mutant complete cds; Interferon receptor 1 bound protein 4; Interferon receptor 1 bound protein4; Interferon receptor 1bound protein 4; IR1 B4; IR1B 4; Mrmt 1; Mrmt1; PRMT 1; Protein arginine N methyltransferase 1; Protein arginine N methyltransferase1; S; cerevisiae like 2.; PRMT1 active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid in 40mM Tris pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT, 40% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR
• Sequence Length: 353

• Applicable Applications for PRMT1 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-MBP tag
• Biological Activity: 10,000unit/ml
• Unit Definition: One unit will transfer 1pmol of methyl group to synthetic; peptide of histone H4 for 10 minutes at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PRMT1 active protein

Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Recombinant human PRMT1, fused to His-MBP tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PRMT1 active protein

Histone Related

References

Goulet I., et al. (2007) J Biol Chem. 282 (45):33009-21.; Tang J., et al. (2000) J Biol Chem. 275 (11):7723-30.;

NCBI and Uniprot Product Information

• NCBI GI #: 151301219
• NCBI GeneID: 3276
• NCBI Accession #: NP_938074
• NCBI GenBank Nucleotide #: NM_198318.4
• UniProt Accession #: Q99873; Q15529; Q2VP93; Q6LEU5; Q8WUW5; Q99872; Q99874; Q9NZ04; Q9NZ05; Q9NZ06; B4E3C3; G5E9B6
• Molecular Weight: 84 kDa (750 aa)
• NCBI Official Full Name: protein arginine N-methyltransferase 1 isoform 3
• NCBI Official Synonym Full Names: protein arginine methyltransferase 1
• NCBI Official Symbol: PRMT1
• NCBI Official Synonym Symbols: ANM1; HCP1; IR1B4; HRMT1L2
• NCBI Protein Information: protein arginine N-methyltransferase 1
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT1
• UniProt Synonym Gene Names: HMT2; HRMT1L2; IR1B4

NCBI Description

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:16879614). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).