Typical Testing Data/Standard Curve (for reference only)
Mouse Nesfatin 1 (NES1) ELISA Kit | NES1 elisa kit
Mouse Mini Samples Nesfatin 1 (NES1) ELISA Kit
Reactivity
Mouse
Synonyms
Nesfatin 1 (NES1); Mouse Mini Samples Nesfatin 1 (NES1) ELISA Kit; NES1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Nesfatin 1 (NES1).
No significant cross-reactivity or interference between Mini Samples Nesfatin 1 (NES1) and analogues was observed.
No significant cross-reactivity or interference between Mini Samples Nesfatin 1 (NES1) and analogues was observed.
Sequence Length
420
Assay Type
Competitive Inhibition
Sample
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
Dectection Range
493.8-40,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 187.7pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Nesfatin 1 (NES1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Nesfatin 1 (NES1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Nesfatin 1 (NES1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NES1 elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Nesfatin 1 (NES1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Nesfatin 1 (NES1) and unlabeled Mini Samples Nesfatin 1 (NES1) (Standards or samples) with the pre-coated antibody specific to Mini Samples Nesfatin 1 (NES1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Nesfatin 1 (NES1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Nesfatin 1 (NES1) in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nucleobindin-2
UniProt Protein Name
Nucleobindin-2
UniProt Gene Name
Nucb2
UniProt Synonym Gene Names
Nefa
UniProt Entry Name
NUCB2_MOUSE
Uniprot Description
NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Vesicle; Secreted
Cellular Component: Golgi apparatus; nuclear outer membrane; extracellular space; membrane; endoplasmic reticulum; cell; cytoplasm; ER-Golgi intermediate compartment; extracellular region; nucleus; Golgi medial cisterna
Molecular Function: DNA binding; metal ion binding; calcium ion binding; tumor necrosis factor receptor binding
Biological Process: cellular calcium ion homeostasis
Similar Products
Product Notes
The Mouse NES1 nucb2 (Catalog #AAA2122226) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2122226 ELISA Kit recognizes Mouse NES1. It is sometimes possible for the material contained within the vial of "Nesfatin 1 (NES1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
