Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-Vb (MYO5B) Standard Curve.)
Mouse Myosin-Vb (MYO5B) ELISA Kit | MYO5B elisa kit
Mouse Myosin-Vb (MYO5B) ELISA Kit
Gene Names
Myo5b; AI661750; mKIAA1119
Reactivity
Mouse
Synonyms
Myosin-Vb (MYO5B); Mouse Myosin-Vb (MYO5B) ELISA Kit; MYO5B; KIAA1119; MYO5B variant protein; MYO5B elisa kit
Reactivity
Mouse
Specificity
Mouse Myosin-Vb (MYO5B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYO5B. No significant cross-reactivity or interference between Mouse MYO5B and analogues was observed.
Sequence Length
1818
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-Vb (MYO5B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-Vb (MYO5B) Standard Curve.)
Related Product Information for MYO5B elisa kit
Description: This Mouse Myosin-Vb (MYO5B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO5B in samples. An antibody specific for MYO5B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO5B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO5B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO5B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.
Background: May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Unconventional myosin-Vb
NCBI Official Synonym Full Names
myosin VB
NCBI Official Symbol
Myo5b
NCBI Official Synonym Symbols
AI661750; mKIAA1119
NCBI Protein Information
unconventional myosin-Vb
UniProt Protein Name
Unconventional myosin-Vb
Protein Family
UniProt Gene Name
Myo5b
UniProt Synonym Gene Names
Kiaa1119
UniProt Entry Name
MYO5B_MOUSE
Research Articles on MYO5B
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Product Notes
The Mouse MYO5B myo5b (Catalog #AAA9717473) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717473 ELISA Kit recognizes Mouse MYO5B. It is sometimes possible for the material contained within the vial of "Myosin-Vb (MYO5B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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