Typical Standard Curve/Testing Data
Mouse Myosin VA (MYO5A) ELISA Kit | MYO5A elisa kit
Mouse Myosin VA (MYO5A) ELISA Kit
Gene Names
Myo5a; d; Dbv; MVa; flr; Myo5; MyoVA; Sev-1; flail; d-120J; AI413174; AI661011; 9630007J19Rik
Reactivity
Mouse
Synonyms
Myosin VA (MYO5A); Mouse Myosin VA (MYO5A) ELISA Kit; GS1; MYH12; MYO5; MYOXIN; MYR12; myosin V; myosin Va; Myosin; Heavy Polypeptide Kinase; Myosin Heavy Chain 12; Myoxin; Myosin V; Dilute myosin heavy chain; non-muscle; MYO5A elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of MYO5A. No significant cross-reactivity or interference between MYO5A and analogues was observed.
Sequence Length
1853
Samples
Tissue homogenates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.045ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYO5A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYO5A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for MYO5A elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MYO5A in mouse tissue homogenates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYO5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MYO5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYO5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MYO5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYO5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MYO5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYO5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MYO5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
215,538 Da
NCBI Official Full Name
unconventional myosin-Va
NCBI Official Synonym Full Names
myosin VA
NCBI Official Symbol
Myo5a
NCBI Official Synonym Symbols
d; Dbv; MVa; flr; Myo5; MyoVA; Sev-1; flail; d-120J; AI413174; AI661011; 9630007J19Rik
NCBI Protein Information
unconventional myosin-Va
UniProt Protein Name
Unconventional myosin-Va
Protein Family
UniProt Gene Name
Myo5a
UniProt Synonym Gene Names
Dilute
Uniprot Description
Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.
Research Articles on MYO5A
Similar Products
Product Notes
The Mouse MYO5A myo5a (Catalog #AAA450614) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA450614 ELISA Kit recognizes Mouse MYO5A. It is sometimes possible for the material contained within the vial of "Myosin VA (MYO5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
