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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Myosin light chain 3 (MYL3) Standard Curve.)

Mouse Myosin Light Chain 3 (MYL3) ELISA Kit | MYL3 elisa kit

Mouse Myosin Light Chain 3 (MYL3) ELISA Kit

Gene Names
Myl3; Mylc; VLC1; MLC1s; MLC1v; MLC1SB
Reactivity
Mouse
Synonyms
Myosin Light Chain 3 (MYL3); Mouse Myosin Light Chain 3 (MYL3) ELISA Kit; MYL3; CMH8; MLC1SB; MLC1V; VLC1; OTTHUMP00000165922; myosin; light polypeptide 3; alkali; ventricular; skeletal; slow; slow skeletal ventricular myosin alkali light chain 3; MYL3 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
Mouse Myosin light chain 3 (MYL3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYL3. No significant cross-reactivity or interference between Mouse MYL3 and analogues was observed.
Sequence Length
204
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Myosin light chain 3 (MYL3) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Mouse Myosin light chain 3 (MYL3) Standard Curve.)
Related Product Information for MYL3 elisa kit
Description: This Mouse Myosin light chain 3 (MYL3) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL3 in samples. An antibody specific for MYL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin light chain 3
NCBI Official Synonym Full Names
myosin, light polypeptide 3
NCBI Official Symbol
Myl3
NCBI Official Synonym Symbols
Mylc; VLC1; MLC1s; MLC1v; MLC1SB
NCBI Protein Information
myosin light chain 3
UniProt Protein Name
Myosin light chain 3
Protein Family
UniProt Gene Name
Myl3
UniProt Synonym Gene Names
MLC1SB

Uniprot Description

MYL3: Regulatory light chain of myosin. Does not bind calcium. Defects in MYL3 are the cause of familial hypertrophic cardiomyopathy type 8 (CMH8). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 9 F2|9 60.69 cM

Cellular Component: A band; I band; myosin complex

Molecular Function: actin monomer binding; calcium ion binding; motor activity

Biological Process: cardiac muscle contraction; regulation of striated muscle contraction; regulation of the force of heart contraction; ventricular cardiac muscle tissue morphogenesis

Similar Products

Product Notes

The Mouse MYL3 myl3 (Catalog #AAA9717495) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717495 ELISA Kit recognizes Mouse MYL3. It is sometimes possible for the material contained within the vial of "Myosin Light Chain 3 (MYL3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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