Rabbit anti-Human RGS5 Polyclonal Antibody | anti-RGS5 antibody

RGS5, NT (RGS5, Regulator of G-protein signaling 5) (FITC)

Cat. Num. AAA6341788

• Gene Names: RGS5; MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RGS5; Polyclonal Antibody; NT (RGS5; Regulator of G-protein signaling 5) (FITC); Regulator of G-protein signaling 5; anti-RGS5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RGS5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RGS5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-53 amino acids from the N-terminal region of human RGS5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RGS5 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RGS5 antibody detected the RGS5 protein (arrow).)

Immunohistochemistry (IHC)

(RGS5 Antibody (N-term) (AP14899a)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RGS5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RGS5 antibody

This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-RGS5 antibody

Antibodies; G Proteins

References

Wang, J., et al. Carcinogenesis 31(10):1755-1761(2010). Li, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13818-13823(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Xiao, B., et al. Clin. Chem. Lab. Med. 47(12):1483-1488(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4506519
• NCBI GeneID: 8490
• NCBI Accession #: NP_003608.1
• NCBI GenBank Nucleotide #: NM_003617.3
• UniProt Accession #: O15539; Q53XA9; Q599J0; Q7LCP5; Q9H3F2
• Molecular Weight: 20,946 Da
• NCBI Official Full Name: regulator of G-protein signaling 5 isoform 1
• NCBI Official Synonym Full Names: regulator of G-protein signaling 5
• NCBI Official Symbol: RGS5
• NCBI Official Synonym Symbols: MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129
• NCBI Protein Information: regulator of G-protein signaling 5; OTTHUMP00000032361; OTTHUMP00000232302
• UniProt Protein Name: Regulator of G-protein signaling 5
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS5
• UniProt Synonym Gene Names: RGS5
• UniProt Entry Name: RGS5_HUMAN

Uniprot Description

RGS5: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, RGS

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cytoplasm; plasma membrane

Molecular Function: GTPase activator activity

Biological Process: signal transduction; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity

Disease: Hypertension, Essential