Western Blot (WB)
(PJA1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).)
Rabbit anti-Human PJA1 Polyclonal Antibody | anti-PJA1 antibody
PJA1, NT (PJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, RING finger protein 70)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Western Blot (WB)
(PJA1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).)
Western Blot (WB)
(PJA1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).)
Immunohistochemistry (IHC)
(PJA1 Antibody (N-term) (AP14811a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PJA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PJA1 Antibody (N-term) (AP14811a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PJA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked mental retardation disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
Uniprot Description
PJA1: Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome. May be involved in protein sorting. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ligase; EC 6.3.2.19
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: cytoplasm
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: protein ubiquitination; protein catabolic process
Research Articles on PJA1
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Product Notes
The PJA1 pja1 (Catalog #AAA642971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PJA1, NT (PJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, RING finger protein 70) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PJA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the PJA1 pja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PJA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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