Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mucosal addressin cell adhesion molecule 1 (MADCAM1) Standard Curve.)
Mouse Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA Kit | MADCAM1 elisa kit
Mouse Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA Kit
Gene Names
Madcam1; AV211525; MAdCAM-1
Reactivity
Mouse
Synonyms
Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1); Mouse Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1) ELISA Kit; MADCAM1; MACAM1; mucosal addressin cell adhesion molecule-1; MADCAM1 elisa kit
Reactivity
Mouse
Specificity
Mouse Mucosal addressin cell adhesion molecule 1 (MADCAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MADCAM1. No significant cross-reactivity or interference between Mouse MADCAM1 and analogues was observed.
Sequence Length
405
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mucosal addressin cell adhesion molecule 1 (MADCAM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Mucosal addressin cell adhesion molecule 1 (MADCAM1) Standard Curve.)
Related Product Information for MADCAM1 elisa kit
Description: This Mouse Mucosal addressin cell adhesion molecule 1 (MADCAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate MADCAM1 in samples. An antibody specific for MADCAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MADCAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MADCAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MADCAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Addressin is an extracellular protein of the endothelium of venules. Addressins are the ligands to the homing receptors of lymphocytes.The task of these ligands and their receptors is to determine which tissue the lymphocyte will enter next. The carry carbohydrates in order to be recognized by L-selectin. The predicted amino acid sequence defines the mucosal addressin as a novel immunoglobulin family member with 2 N-terminal domains that display strong homology to previously described vascular adhesion receptors for leukocytes: ICAM1 and VCAM1. The membrane proximal domain is homologous to the third domain of another mucosa-associated member of the immunoglobulin family, namely, IgA0.
Background: Addressin is an extracellular protein of the endothelium of venules. Addressins are the ligands to the homing receptors of lymphocytes.The task of these ligands and their receptors is to determine which tissue the lymphocyte will enter next. The carry carbohydrates in order to be recognized by L-selectin. The predicted amino acid sequence defines the mucosal addressin as a novel immunoglobulin family member with 2 N-terminal domains that display strong homology to previously described vascular adhesion receptors for leukocytes: ICAM1 and VCAM1. The membrane proximal domain is homologous to the third domain of another mucosa-associated member of the immunoglobulin family, namely, IgA0.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Mucosal addressin cell adhesion molecule 1
NCBI Official Synonym Full Names
mucosal vascular addressin cell adhesion molecule 1
NCBI Official Symbol
Madcam1
NCBI Official Synonym Symbols
AV211525; MAdCAM-1
NCBI Protein Information
mucosal addressin cell adhesion molecule 1
UniProt Protein Name
Mucosal addressin cell adhesion molecule 1
Protein Family
UniProt Gene Name
Madcam1
UniProt Synonym Gene Names
MAdCAM-1; mMAdCAM-1
UniProt Entry Name
MADCA_MOUSE
Uniprot Description
MADCAM1: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Cellular Component: membrane; integral to membrane
Biological Process: keratinocyte differentiation; positive regulation of leukocyte migration; cell adhesion; leukocyte migration
Research Articles on MADCAM1
Similar Products
Product Notes
The Mouse MADCAM1 madcam1 (Catalog #AAA9717752) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717752 ELISA Kit recognizes Mouse MADCAM1. It is sometimes possible for the material contained within the vial of "Mucosal Addressin Cell Adhesion Molecule 1 (MADCAM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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