Human Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) ELISA Kit | MAdCAM1 elisa kit

Human Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) ELISA Kit

Cat. Num. AAA2021711

• Gene Names: MADCAM1; MACAM1
• Reactivity: Human
• Synonyms: Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1); Human Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) ELISA Kit; Addressin; MAdCAM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1).; No significant cross-reactivity or interference between Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) and analogues was observed.
• Sequence Length: 200

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Urine and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 37pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAdCAM1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MAdCAM1 elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 109633022
• NCBI GeneID: 8174
• NCBI Accession #: NP_570116.2
• NCBI GenBank Nucleotide #: NM_130760.2
• UniProt Accession #: Q13477; O60222; O75867; Q5UGI7; A5PKV4; B2RPL9
• Molecular Weight: 21,247 Da
• NCBI Official Full Name: mucosal addressin cell adhesion molecule 1 isoform a
• NCBI Official Synonym Full Names: mucosal vascular addressin cell adhesion molecule 1
• NCBI Official Symbol: MADCAM1
• NCBI Official Synonym Symbols: MACAM1
• NCBI Protein Information: mucosal addressin cell adhesion molecule 1
• UniProt Protein Name: Mucosal addressin cell adhesion molecule 1
• Protein Family: Mucosal addressin cell adhesion molecule
• UniProt Gene Name: MADCAM1
• UniProt Synonym Gene Names: MAdCAM-1; hMAdCAM-1
• UniProt Entry Name: MADCA_HUMAN

NCBI Description

The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MADCAM1: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; plasma membrane; integral to membrane

Biological Process: integrin-mediated signaling pathway; regulation of immune response; extracellular matrix organization and biogenesis; positive regulation of leukocyte migration; cell-matrix adhesion; signal transduction; receptor clustering; keratinocyte differentiation; heterotypic cell-cell adhesion; immune response; cell adhesion; leukocyte tethering or rolling; aging