Rat Mothers Against Decapentaplegic Homolog 7 (Smad7) ELISA Kit | Smad7 elisa kit

Rat Mothers Against Decapentaplegic Homolog 7 (Smad7) ELISA Kit

Cat. Num. AAA2020338

• Gene Names: Smad7; Madh7
• Reactivity: Rat
• Synonyms: Mothers Against Decapentaplegic Homolog 7 (Smad7); Rat Mothers Against Decapentaplegic Homolog 7 (Smad7) ELISA Kit; MADH7; MADH8; Mothers against DPP homolog 8; Smad7 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 7 (Smad7).; No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 7 (Smad7) and analogues was observed.
• Sequence Length: 426

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 7 (Smad7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 7 (Smad7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Smad7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 7 (Smad7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 7 (Smad7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 7 (Smad7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 7 (Smad7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Smad7 elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 111154105
• NCBI GeneID: 17131
• NCBI Accession #: NP_001036125.1
• NCBI GenBank Nucleotide #: NM_001042660.1
• UniProt Accession #: O35253; O88709
• Molecular Weight: 46,371 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 7
• NCBI Official Synonym Full Names: SMAD family member 7
• NCBI Official Symbol: Smad7
• NCBI Official Synonym Symbols: Madh7
• NCBI Protein Information: mothers against decapentaplegic homolog 7
• UniProt Protein Name: Mothers against decapentaplegic homolog 7
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: Smad7
• UniProt Synonym Gene Names: Madh7; Madh8; MAD homolog 7; Mothers against DPP homolog 7; MAD homolog 8; Mothers against DPP homolog 8; SMAD 7; Smad7
• UniProt Entry Name: SMAD7_MOUSE

Uniprot Description

SMAD7: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Genetic variations in SMAD7 influence susceptibility to colorectal cancer type 3 (CRCS3). Colorectal cancer consists of tumors or cancer of either the colon or rectum or both. Cancers of the large intestine are the second most common form of cancer found in males and females. Symptoms include rectal bleeding, occult blood in stools, bowel obstruction and weight loss. Treatment is based largely on the extent of cancer penetration into the intestinal wall. Surgical cures are possible if the malignancy is confined to the intestine. Risk can be reduced when following a diet which is low in fat and high in fiber. Belongs to the dwarfin/SMAD family.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; cell-cell adherens junction; protein complex; cytoplasm; plasma membrane; intracellular; catenin complex; nucleus

Molecular Function: collagen binding; protein binding; DNA binding; ubiquitin protein ligase binding; metal ion binding; beta-catenin binding; activin binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; transcription factor activity

Biological Process: transcription, DNA-dependent; protein stabilization; negative regulation of transcription factor activity; negative regulation of peptidyl-serine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; regulation of activin receptor signaling pathway; negative regulation of BMP signaling pathway; regulation of transforming growth factor beta receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of protein ubiquitination; transforming growth factor beta receptor signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity; positive regulation of cell-cell adhesion; artery morphogenesis; ventricular cardiac muscle morphogenesis; negative regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway