Human Neprilysin Assay Kit | MME assay kit

Neprilysin Inhibitor Screening Kit (Fluorometric)

Cat. Num. AAA846926

• Gene Names: MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
• Reactivity: Human
• Synonyms: Neprilysin; Neprilysin Inhibitor Screening Kit (Fluorometric); MME assay kit

• Reactivity: Human
• Sequence Length: 750

• Application Notes: Screening/characterizing/studying Neprilysin inhibitors/activators
• Preparation and Storage: Store at -20 degree C for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MME assay kit

Background/Introduction: Neprilysin (NEP, EC 3.4.24.11), also known as neutral endopeptidase, enkephalinase, CD10, and common acute lymphoblastic leukemia antigen, is a type II zinc-containing transmembrane metalloproteinase. It is able to hydrolyze very important endogenous peptides, such as natriuretic atrial factor, enkephalins, substance P, bradykinin and amyloid beta-peptide. Thus, NEP is a potentially therapeutic target in important pathological conditions such as cardiovascular disease, prostate cancer, Alzheimer's disease and analgesia. NEP has also been used as a biological marker of a type of childhood leukemia called CALLA. NEP is currently a focus of interest in cardiovascular and neurobiological research. NEP inhibitors have shown promising results as potential treatment alternative for some conditions including hypertension, heart failure, renal failure, and improvement of beta-cell function in obese type 2 diabetes mellitus. The combination of NEP inhibitor and angiotensin II type 1 receptor blockade has been used clinically to treat patients with chronic heart failure. The Neprilysin Inhibitor Screening Kit can be used to screen for potential inhibitors of NEP activity. It utilizes the ability of an active NEP to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader. In the presence of an NEP specific inhibitor, the enzyme loses its activity which results in decrease of fluorescence intensity. The assay kit is simple and can be used to identify and characterize NEP inhibitors in a high-throughput format.

Product Categories/Family for MME assay kit

Proteases

NCBI and Uniprot Product Information

• NCBI GI #: 116256333
• NCBI GeneID: 4311
• NCBI Accession #: NP_009220.2
• NCBI GenBank Nucleotide #: NM_007289.2
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: 85,514 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metalloendopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; CMT2T; SCA43
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: Neprilysin
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE

NCBI Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: EC 3.4.24.11; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: axon; brush border; cytoplasm; dendrite; focal adhesion; plasma membrane; secretory granule membrane; synapse; synaptic vesicle

Molecular Function: endopeptidase activity; exopeptidase activity; metalloendopeptidase activity; metallopeptidase activity; peptide binding; protein binding; zinc ion binding

Biological Process: angiotensin maturation; beta-amyloid metabolic process; creatinine metabolic process; kidney development; neutrophil degranulation; peptide metabolic process; proteolysis; sensory perception of pain

Disease: Charcot-marie-tooth Disease, Axonal, Type 2t; Membranous Nephropathy, Susceptibility To; Spinocerebellar Ataxia 43