Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Metastasis-suppressor KiSS-1 (KISS1) Standard Curve.)
Mouse Metastasis-Suppressor KiSS-1 (KISS1) ELISA Kit | KISS1 elisa kit
Mouse Metastasis-Suppressor KiSS-1 (KISS1) ELISA Kit
Gene Names
Kiss1; kisspeptin; metastatin
Reactivity
Mouse
Synonyms
Metastasis-Suppressor KiSS-1 (KISS1); Mouse Metastasis-Suppressor KiSS-1 (KISS1) ELISA Kit; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor; KISS1 elisa kit
Reactivity
Mouse
Specificity
Mouse Metastasis-suppressor KiSS-1 (KISS1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse KISS1. No significant cross-reactivity or interference between Mouse KISS1 and analogues was observed.
Sequence Length
126
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Metastasis-suppressor KiSS-1 (KISS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Metastasis-suppressor KiSS-1 (KISS1) Standard Curve.)
Related Product Information for KISS1 elisa kit
Description: This Mouse Metastasis-suppressor KiSS-1 (KISS1) ELISA Kit employs a two-site sandwich ELISA to quantitate KISS1 in samples. An antibody specific for KISS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KISS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KISS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KISS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Kisspeptin is a G-protein coupled receptor ligand for GPR54. Kiss1 was originally identified as a human metastasis suppressor gene that has the ability to suppress melanoma and breast cancer metastasis. It is recently become clear that kisspeptin-GPR54 signaling has an important role in initiating GnRH secretion at puberty, the extent of which is an area of ongoing research.Kisspeptin is a metastasis suppressor of melanomas and breast cancers without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms.
Background: Kisspeptin is a G-protein coupled receptor ligand for GPR54. Kiss1 was originally identified as a human metastasis suppressor gene that has the ability to suppress melanoma and breast cancer metastasis. It is recently become clear that kisspeptin-GPR54 signaling has an important role in initiating GnRH secretion at puberty, the extent of which is an area of ongoing research.Kisspeptin is a metastasis suppressor of melanomas and breast cancers without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
metastasis-suppressor KiSS-1
NCBI Official Synonym Full Names
KiSS-1 metastasis-suppressor
NCBI Official Symbol
Kiss1
NCBI Official Synonym Symbols
kisspeptin; metastatin
NCBI Protein Information
metastasis-suppressor KiSS-1
UniProt Protein Name
Metastasis-suppressor KiSS-1
Protein Family
UniProt Gene Name
Kiss1
Uniprot Description
Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.
Research Articles on KISS1
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Product Notes
The Mouse KISS1 kiss1 (Catalog #AAA9717820) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717820 ELISA Kit recognizes Mouse KISS1. It is sometimes possible for the material contained within the vial of "Metastasis-Suppressor KiSS-1 (KISS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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