Typical Testing Data/Standard Curve (for reference only)
Mouse Calcitonin (CT) ELISA Kit | CT elisa kit
Mouse Calcitonin (CT) ELISA Kit
Gene Names
Calca; CA; Ct; Ctn; Calc; Cgrp; CGRP1; Calc1; CGRP-1
Reactivity
Mouse
Synonyms
Calcitonin (CT); Mouse Calcitonin (CT) ELISA Kit; CALCA; CALC1; CGRP-I; CGRP1; KC; CCP; PDN-21; Katacalcin; Calcitonin-Related Polypeptide Alpha; Calcitonin carboxyl-terminal peptide; CT elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CT. No significant cross-reactivity or interference between CT and analogues was observed.
Sequence Length
136
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Competitive inhibition
Detection Range
15.625-1000pg/mL
Sensitivity
4.45pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CT elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CT in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,065 Da
NCBI Official Full Name
calcitonin gene-related peptide 1 isoform Calca preproprotein
NCBI Official Synonym Full Names
calcitonin/calcitonin-related polypeptide, alpha
NCBI Official Symbol
Calca
NCBI Official Synonym Symbols
CA; Ct; Ctn; Calc; Cgrp; CGRP1; Calc1; CGRP-1
NCBI Protein Information
calcitonin gene-related peptide 1
UniProt Protein Name
Calcitonin
UniProt Gene Name
Calca
UniProt Synonym Gene Names
Calc
NCBI Description
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide (CGRP) and katacalcin. Alternative splicing of the mRNA results in multiple variants that encode either calcitonin or CGRP preproproteins. Post-translational processing of the calcitonin and CGRP propeptides results in either calcitonin and katacalcin, or CGRP, respectively. Calcitonin and katacalcin modulate calcium levels in the blood stream. CGRP can function as a vasodilator and play a role in the transmission of pain. The human homolog of CGRP was found to have antimicrobial activity. [provided by RefSeq, Mar 2015]
Uniprot Description
Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Research Articles on CT
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Product Notes
The Mouse CT calca (Catalog #AAA450148) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA450148 ELISA Kit recognizes Mouse CT. It is sometimes possible for the material contained within the vial of "Calcitonin (CT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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