Typical Testing Data/Standard Curve (for reference only)
Mouse macrophage migration inhibitory factor (glycosylation-inhibiting factor) ELISA Kit | MIF elisa kit
Mouse Macrophage Migration Inhibitory Factor, MIF ELISA Kit
Gene Names
Mif; GIF; Glif
Reactivity
Mouse
Synonyms
macrophage migration inhibitory factor (glycosylation-inhibiting factor); Mouse Macrophage Migration Inhibitory Factor; MIF ELISA Kit; GIF; GLIF; MMIF; macrophage migration inhibitory factor; phenylpyruvate tautomerase; MIF elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse MIF. No significant cross-reactivity or interference between mouse MIF and analogues was observed.
Sequence Length
115
Samples
Serum, plasma, cell culture supernates and tissue homogenates
Assay Type
Sandwich
Detection Range
0.625 pg/ml -40 pg/ml.
Sensitivity
The minimum detectable dose of mouse MIF is typically less than 0.156 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MIF elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,504 Da
NCBI Official Full Name
macrophage migration inhibitory factor
NCBI Official Synonym Full Names
macrophage migration inhibitory factor
NCBI Official Symbol
Mif
NCBI Official Synonym Symbols
GIF; Glif
NCBI Protein Information
macrophage migration inhibitory factor; DER6; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; glycosylation-inhibiting factor; delayed early response protein 6
UniProt Protein Name
Macrophage migration inhibitory factor
Protein Family
UniProt Gene Name
Mif
UniProt Synonym Gene Names
MIF; DER6; GIF
UniProt Entry Name
MIF_MOUSE
Uniprot Description
Function: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity
Catalytic activity: Keto-phenylpyruvate = enol-phenylpyruvate.1 PublicationManual assertion based on experiment in:Ref.12
Research Articles on MIF
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Product Notes
The Mouse MIF mif (Catalog #AAA701017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701017 ELISA Kit recognizes Mouse MIF. It is sometimes possible for the material contained within the vial of "macrophage migration inhibitory factor (glycosylation-inhibiting factor), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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