Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) Standard Curve.)
Mouse Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase (LIPA) ELISA Kit | LIPA elisa kit
Mouse Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase (LIPA) ELISA Kit
Gene Names
Lipa; Lal; Lip1; Lip-1; AA960673
Reactivity
Mouse
Synonyms
Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase (LIPA); Mouse Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase (LIPA) ELISA Kit; LIPA; CESD; LAL; OTTHUMP00000020068; cholesterol ester hydrolase; lipase A; lysosomal acid lipase; sterol esterase; LIPA elisa kit
Reactivity
Mouse
Specificity
Mouse Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LIPA. No significant cross-reactivity or interference between Mouse LIPA and analogues was observed.
Sequence Length
397
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) Standard Curve.)
Related Product Information for LIPA elisa kit
Description: This Mouse Lysosomal acid lipase/cholesteryl ester hydrolase (LIPA) ELISA Kit employs a two-site sandwich ELISA to quantitate LIPA in samples. An antibody specific for LIPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lysosomal acid lipase (LIPA, or LAL), otherwise known as acid cholesteryl ester hydrolase, is coded for by a gene (LIPA) on chromosome 10. Two major disorders, the severe infantile-onset Wolman disease and the milder late-onset cholesteryl ester storage disease (CESD), are seemingly caused by mutations in different parts of the LIPA gene. Aslanidis et al. (1996) provided evidence that a strikingly more severe course of Wolman disease is caused by genetic defects of LAL that leave no residual enzyme activity. In a CESD patient, a G-to-A transition at position -1 of the exon 8 splice donor site resulted in skipping of exon 8 in 97% of the mRNA originating from this allele, while 3% was spliced correctly, resulting in full-length LAL enzyme.
Background: Lysosomal acid lipase (LIPA, or LAL), otherwise known as acid cholesteryl ester hydrolase, is coded for by a gene (LIPA) on chromosome 10. Two major disorders, the severe infantile-onset Wolman disease and the milder late-onset cholesteryl ester storage disease (CESD), are seemingly caused by mutations in different parts of the LIPA gene. Aslanidis et al. (1996) provided evidence that a strikingly more severe course of Wolman disease is caused by genetic defects of LAL that leave no residual enzyme activity. In a CESD patient, a G-to-A transition at position -1 of the exon 8 splice donor site resulted in skipping of exon 8 in 97% of the mRNA originating from this allele, while 3% was spliced correctly, resulting in full-length LAL enzyme.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lysosomal acid lipase/cholesteryl ester hydrolase
NCBI Official Synonym Full Names
lysosomal acid lipase A
NCBI Official Symbol
Lipa
NCBI Official Synonym Symbols
Lal; Lip1; Lip-1; AA960673
NCBI Protein Information
lysosomal acid lipase/cholesteryl ester hydrolase
UniProt Protein Name
Lysosomal acid lipase/cholesteryl ester hydrolase
Protein Family
UniProt Gene Name
Lipa
UniProt Synonym Gene Names
Lip1; Acid cholesteryl ester hydrolase; LAL
Uniprot Description
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation ().
Research Articles on LIPA
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Product Notes
The Mouse LIPA lipa (Catalog #AAA9717782) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717782 ELISA Kit recognizes Mouse LIPA. It is sometimes possible for the material contained within the vial of "Lysosomal Acid Lipase/Cholesteryl Ester Hydrolase (LIPA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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