Typical Testing Data/Standard Curve (for reference only)
Mouse Lymphocyte Antigen 75 (LY75) ELISA Kit | LY75 elisa kit
Mouse Lymphocyte Antigen 75 (LY75) ELISA Kit
Gene Names
LY75; CD205; LY-75; CLEC13B; DEC-205; GP200-MR6
Reactivity
Mouse
Synonyms
Lymphocyte Antigen 75 (LY75); Mouse Lymphocyte Antigen 75 (LY75) ELISA Kit; CD205; LY75; CLEC13B; DEC-205; GP200-MR6; C-type lectin domain family 13 member B; LY75 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 75 (LY75).
No significant cross-reactivity or interference between Lymphocyte Antigen 75 (LY75) and analogues was observed.
No significant cross-reactivity or interference between Lymphocyte Antigen 75 (LY75) and analogues was observed.
Sequence Length
232
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Lymphocyte Antigen 75 (LY75) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LY75 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 75 (LY75). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 75 (LY75). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 75 (LY75), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Antigen 75 (LY75) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for LY75 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,183 Da
NCBI Official Full Name
lymphocyte antigen 75
NCBI Official Synonym Full Names
lymphocyte antigen 75
NCBI Official Symbol
LY75
NCBI Official Synonym Symbols
CD205; LY-75; CLEC13B; DEC-205; GP200-MR6
NCBI Protein Information
lymphocyte antigen 75
UniProt Protein Name
Lymphocyte antigen 75
Protein Family
UniProt Gene Name
LY75
UniProt Synonym Gene Names
CD205; CLEC13B; Ly-75
UniProt Entry Name
LY75_HUMAN
Uniprot Description
LY75: Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen- processing compartment. Causes reduced proliferation of B-lymphocytes. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q24
Cellular Component: integral to plasma membrane
Molecular Function: receptor activity; carbohydrate binding
Biological Process: endocytosis; immune response; inflammatory response
Research Articles on LY75
Similar Products
Product Notes
The Mouse LY75 ly75 (Catalog #AAA2024175) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024175 ELISA Kit recognizes Mouse LY75. It is sometimes possible for the material contained within the vial of "Lymphocyte Antigen 75 (LY75), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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