Rabbit anti-Rat HGF Polyclonal Antibody | anti-HGF antibody

Anti-HGF Antibody, Rabbit Polyclonal

Cat. Num. AAA8113012

• Gene Names: Hgf; HPTA
• Reactivity: Rat
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: HGF; Polyclonal Antibody; Anti-HGF Antibody; Rabbit Polyclonal; HGF/Hepatocyte Growth Factor Antibody; Rabbit PAb; Antigen Affinity Purified; hepatocyte growth factor (hepapoietin A; scatter factor); anti-HGF antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat HGF/Hepatocyte Growth Factor
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HGF antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:200-1:1000
• Immunogen: Recombinant Rat HGF/Hepatocyte Growth Factor Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat HGF/Hepatocyte Growth Factor (rR HGF/Hepatocyte Growth Factor). HGF/Hepatocyte Growth Factor specific IgG was purified by Rat HGF/Hepatocyte Growth Factor affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-HGF rabbit polyclonal antibody at 1:500 dilution Lane A: Rat liver tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:83 kDa Observed band size:93 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of rat HGF in rat liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of rat HGF in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-HGF antibody

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Product Categories/Family for anti-HGF antibody

Cancer Drug Targets

References

Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.

NCBI and Uniprot Product Information

• NCBI GI #: 8393526
• NCBI GeneID: 24446
• NCBI Accession #: NP_058713.1
• NCBI GenBank Nucleotide #: NM_017017.2
• UniProt Accession #: P17945
• Molecular Weight: 82,906 Da
• NCBI Official Full Name: hepatocyte growth factor preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor
• NCBI Official Symbol: Hgf
• NCBI Official Synonym Symbols: HPTA
• NCBI Protein Information: hepatocyte growth factor
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: Hgf
• UniProt Synonym Gene Names: SF
• UniProt Entry Name: HGF_RAT

NCBI Description

plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle [RGD, Feb 2006]

Uniprot Description

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization ().