Mouse Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit | LECT1 elisa kit

Mouse Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit

Cat. Num. AAA450336

• Gene Names: Cnmd; Chmd; ChM-I; Lect1; Bricd3
• Reactivity: Mouse
• Synonyms: Leukocyte Cell Derived Chemotaxin 1 (LECT1); Mouse Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit; BRICD3; CHM-I; CHM1; CH-SP; Chondromodulin-1; Chondrosurfactant protein; LECT1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of LECT1. No significant cross-reactivity or interference between LECT1 and analogues was observed.
• Sequence Length: 338

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 11.3pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LECT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LECT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LECT1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LECT1 in mouse serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LECT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LECT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LECT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LECT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 893571730
• NCBI GeneID: 16840
• NCBI Accession #: NP_001297584.1
• NCBI GenBank Nucleotide #: NM_001310655.1
• UniProt Accession #: Q9Z1F6; Q80UX1; Q9CXU5
• Molecular Weight: 37,225 Da
• NCBI Official Full Name: leukocyte cell-derived chemotaxin 1 isoform 1
• NCBI Official Synonym Full Names: chondromodulin
• NCBI Official Symbol: Cnmd
• NCBI Official Synonym Symbols: Chmd; ChM-I; Lect1; Bricd3
• NCBI Protein Information: leukocyte cell-derived chemotaxin 1
• UniProt Protein Name: Leukocyte cell-derived chemotaxin 1
• Protein Family: Leukocyte cell-derived chemotaxin
• UniProt Gene Name: Lect1
• UniProt Synonym Gene Names: Chmi; CH-SP; ChM-I

Uniprot Description

Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development (). Inhibits in vitro tube formation and mobilization of endothelial cells (). Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.