Human Leukocyte Cell Derived Chemotaxin 1 (LECT1) RTU ELISA Kit | LECT1 rtu elisa kit

Human Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit

Cat. Num. AAA4500722

• Gene Names: CNMD; CHM1; CHM-I; LECT1; BRICD3; MYETS1
• Reactivity: Human
• Synonyms: Leukocyte Cell Derived Chemotaxin 1 (LECT1); Human Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit; BRICD3; CHM-I; CHM1; CH-SP; Chondromodulin-1; Chondrosurfactant protein; LECT1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 333

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.051ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LECT1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LECT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LECT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LECT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LECT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 59806343
• NCBI GeneID: 11061
• NCBI Accession #: NP_001011705.1
• NCBI GenBank Nucleotide #: NM_001011705.1
• UniProt Accession #: O75829; Q5TAM4; Q8TAY6; Q9UM18
• Molecular Weight: 36,974 Da
• NCBI Official Full Name: leukocyte cell-derived chemotaxin 1 isoform 2
• NCBI Official Synonym Full Names: chondromodulin
• NCBI Official Symbol: CNMD
• NCBI Official Synonym Symbols: CHM1; CHM-I; LECT1; BRICD3; MYETS1
• NCBI Protein Information: leukocyte cell-derived chemotaxin 1
• UniProt Protein Name: Leukocyte cell-derived chemotaxin 1
• Protein Family: Leukocyte cell-derived chemotaxin
• UniProt Gene Name: CNMD
• UniProt Synonym Gene Names: CH-SP; ChM-I

NCBI Description

This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.