Mouse Lactate Dehydrogenase B (LDHB) ELISA Kit | LDHB elisa kit

Mouse Lactate Dehydrogenase B (LDHB) ELISA Kit

Cat. Num. AAA453718

• Gene Names: Ldhb; Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582
• Reactivity: Mouse
• Synonyms: Lactate Dehydrogenase B (LDHB); Mouse Lactate Dehydrogenase B (LDHB) ELISA Kit; LDH-B; LDH-H; Renal carcinoma antigen NY-REN-46; LDHB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of LDHB. No significant cross-reactivity or interference between LDHB and analogues was observed.
• Sequence Length: 259

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.065ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LDHB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LDHB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDHB elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LDHB in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LDHB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LDHB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 718551069
• NCBI GeneID: 16832
• NCBI Accession #: NP_001289694.1
• NCBI GenBank Nucleotide #: NM_001302765.1
• UniProt Accession #: P16125; Q545Y4
• Molecular Weight: 36,572 Da
• NCBI Official Full Name: L-lactate dehydrogenase B chain isoform 2
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: Ldhb
• NCBI Official Synonym Symbols: Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582
• NCBI Protein Information: L-lactate dehydrogenase B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldhb
• UniProt Synonym Gene Names: Ldh-2; Ldh2; LDH-B; LDH-H

NCBI Description

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes 1 and 19. [provided by RefSeq, Feb 2016]