Human LDHB (Lactate Dehydrogenase B) CLIA Kit | LDHB clia kit

Human LDHB (Lactate Dehydrogenase B) CLIA Kit

Cat. Num. AAA2532282

• Gene Names: LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• Reactivity: Human
• Synonyms: LDHB (Lactate Dehydrogenase B); Human LDHB (Lactate Dehydrogenase B) CLIA Kit; LDH-B; LDH-H; LDHB clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant LDHB. No significant cross-reactivity or interference between LDHB and analogues was observed.
• Sequence Length: 334

• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LDHB clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of LDHB concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to LDHB. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LDHB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LDHB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of LDHB. You can calculate the concentration of LDHB in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1200083
• NCBI GeneID: 3945
• UniProt Accession #: P07195
• Molecular Weight: 36,638 Da
• NCBI Official Full Name: lactate dehydrogenase B
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: LDHB
• NCBI Official Synonym Symbols: LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• NCBI Protein Information: L-lactate dehydrogenase B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHB
• UniProt Synonym Gene Names: LDH-B; LDH-H
• UniProt Entry Name: LDHB_HUMAN

NCBI Description

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Recent studies have shown that two protein isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, and that these C-terminally distinct isoforms have different subcellular localization. Alternatively spliced transcript variants have also been found for this gene. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Oct 2015]