Mouse Kallikrein 3 (KLK3) ELISA Kit | KLK3 elisa kit

Mouse Kallikrein 3 (KLK3) ELISA Kit

Cat. Num. AAA453842

• Gene Names: Klkb1; APS; PSA; Kal3; Klk3; Kal-3
• Reactivity: Mouse
• Synonyms: Kallikrein 3 (KLK3); Mouse Kallikrein 3 (KLK3) ELISA Kit; CAP; PLAP; OTASE; IRAP; Oxytocinase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Placental leucine aminopeptidase; KLK3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of KLK3. No significant cross-reactivity or interference between KLK3 and analogues was observed.
• Sequence Length: 638

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KLK3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KLK3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KLK3 in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KLK3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KLK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KLK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 236465805
• NCBI GeneID: 16621
• NCBI Accession #: NP_032481.2
• NCBI GenBank Nucleotide #: NM_008455.3
• UniProt Accession #: P26262; Q8R0P5
• Molecular Weight: 71,383 Da
• NCBI Official Full Name: plasma kallikrein preproprotein
• NCBI Official Synonym Full Names: kallikrein B, plasma 1
• NCBI Official Symbol: Klkb1
• NCBI Official Synonym Symbols: APS; PSA; Kal3; Klk3; Kal-3
• NCBI Protein Information: plasma kallikrein
• UniProt Protein Name: Plasma kallikrein
• Protein Family: Glandular kallikrein
• UniProt Gene Name: Klkb1
• UniProt Synonym Gene Names: Klk3; Pk

NCBI Description

This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric enzyme comprised of heavy and light chains. A complete deletion of the encoded protein prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. [provided by RefSeq, May 2016]

Uniprot Description

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.