Typical Testing Data/Standard Curve (for reference only)
Mouse plasma kallikrein (KLKB1) ELISA Kit | KLKB1 elisa kit
Mouse plasma kallikrein (KLKB1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: KLKB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-KLKB1 antibody and an KLKB1-HRP conjugate. The assay sample and buffer are incubated together with KLKB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLKB1 concentration since KLKB1 from samples and KLKB1-HRP conjugate compete for the anti-KLKB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLKB1 from the sample, fewer sites are left to bind KLKB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLKB1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.34
Cellular Component: extracellular space; extracellular region
Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity
Biological Process: fibrinolysis; hemostasis; positive regulation of fibrinolysis; inflammatory response; blood coagulation; proteolysis; plasminogen activation
Research Articles on KLKB1
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Product Notes
The Mouse KLKB1 klkb1 (Catalog #AAA7242129) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7242129 ELISA Kit recognizes Mouse KLKB1. It is sometimes possible for the material contained within the vial of "plasma kallikrein (KLKB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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