Mouse Islet Amyloid Polypeptide (IAPP) ELISA Kit | IAPP elisa kit

Mouse Islet Amyloid Polypeptide (IAPP) ELISA Kit

Cat. Num. AAA2023200

• Gene Names: IAPP; DAP; IAP
• Reactivity: Mouse
• Synonyms: Islet Amyloid Polypeptide (IAPP); Mouse Islet Amyloid Polypeptide (IAPP) ELISA Kit; IAPP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IAPP. No significant cross-reactivity or interference between IAPP and analogues was observed.
• Sequence Length: 89

• Samples: Mouse serum, plasma and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of IAPP is typically less than 2.7pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IAPP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IAPP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IAPP in mouse serum, plasma and other biological fluids.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Related Product Information for IAPP elisa kit

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IAPP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IAPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IAPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557655
• NCBI GeneID: 3375
• NCBI Accession #: NP_000406.1
• NCBI GenBank Nucleotide #: NM_000415.2
• UniProt Accession #: P10997; Q0ZD87; Q14598
• Molecular Weight: 9,806 Da
• NCBI Official Full Name: islet amyloid polypeptide
• NCBI Official Synonym Full Names: islet amyloid polypeptide
• NCBI Official Symbol: IAPP
• NCBI Official Synonym Symbols: DAP; IAP
• NCBI Protein Information: islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin)
• UniProt Protein Name: Islet amyloid polypeptide
• Protein Family: Islet amyloid polypeptide
• UniProt Gene Name: IAPP
• UniProt Synonym Gene Names: DAP
• UniProt Entry Name: IAPP_HUMAN

NCBI Description

The protein encoded by this gene is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. Studies suggest that this protein, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. This protein also exhibits an bactericidal, antimicrobial activity. [provided by RefSeq, Sep 2014]

Uniprot Description

IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extracellular space; cell soma; extracellular region

Molecular Function: identical protein binding; hormone activity; receptor binding

Biological Process: negative regulation of cell differentiation; cell-cell signaling; eating behavior; apoptosis; signal transduction; sensory perception of pain; negative regulation of bone resorption; endocrine pancreas development